Norbin

Norbin, also known as Neurochondrin, is a neuron-specific cytoplasmic protein involved in dendrite outgrowth. Overexpression of norbin in cultured Neuro 2a cells induced neurite-outgrowth (1). Norbin is located at rat chromosome 5q36. In addition to its involvement in dendrite outgrowth, Norbin is implicated in neural plasticity, chondrocyte differentiation, and early embryogenesis and in bone metabolism by inducing bone marrow cells to absorb bone matrix (2). Norbin is also an important endogenous modulator of metabotropic glutamate receptor 5 (mGluR5). It increases the cell surface localization of the receptor and positively regulates its signaling by physically interacting with the receptor (3). Norbin also acts as a signal molecule. It is a negative regulator of Ca²⁺/Calmodulin-dependent protein kinase II (CaMKII) phosphorylation and it is essential for the spatial learning process of the neurons (4).

Recently it was shown that Norbin is a Diaphanous1 (Dia1) interacting protein, specifically binding to only the formin homology 3 (FH3). Furthermore, it was uncovered that the first 100 amino acids, but not the C-terminal region, of Norbin were responsible for its effect on neurite outgrowth (5). Norbin knockout mice showed a behavioral phenotype associated with a rodent model of schizophrenia and disruption of norbin gene resulted in epileptic seizure (3). Targeted disruption of Norbin gene resulted in embryonic lethality (6). It is also suggested that Norbin is involved in age and sex dependent brain function. Norbin is expressed in hippocampus, amygdala, septum, nucleus accumbens and dorsal striatum. It is approximately an 87.5kDa protein (729 amino acids).

The NORB-selective antibodies were generated against purified Slingshot1 isoform1 (NORB) protein. NORB-antibodies are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. FabGennix Int. Inc. will also provide limited quantities of antigenic blocking protein for NORB -101AP antibody. Antibodies to NORB (NORB-101AP) will label ~87.5kDa protein in Western blot positive control samples for NORB and several other tissues. FabGennix Inc. will also conjugate antibodies with secondary enzymes (alk-Pase or HRP) or fluorescent probes upon request at nominal cost.

For research use only, not for diagnostic or therapeutic use.