PR domain containing 9

PR domain containing 9 protein (PRDM9) is one of at least 20 PR domain containing molecules in human (1). The PR domain is a derivative of SET domain which has been known to act as catalytic domain for histone lysine methylation (2). PRDM9 is located on human chromosome 5p14, it contains a PR domain in its N-terminal portion and a C₂H₂ type zinc finger motif in its C-terminal portion (3). A PR domain’s catalytic activity for histone methylation is dependent upon the individual proteins of that PR domain. The catalytic activity of PRDM9 towards histone H3 is restricted only for tri-methylation of lysine 4 in histone H3 (H3K4) from di-methylation (4). Tri-methylation of H3K4 is a characteristic trait of transcriptionally active genes (5); therefore, PRDM9 is often implicated in transcriptional regulation. PRDM9 also plays an essential role on proper progression of meiotic prophase in mouse germ cell lineage (4).

Recent study has reported that PRDM9 is expressed in early meiosis and it controls the activation of mammalian recombination hotspots (6). Mammalian meiotic recombination occurs at these hotspots, ensuring the orderly segregation of meiotic chromosome and thus creating genetic variation among offspring. Therefore, deficiency of PRDM9 in mice resulted in sterility in both sexes (6). PRDM9 mutations in human can cause failure in spermatogenesis and thus idiopathic infertility in human males (7). PRDM9 is expressed in germ cells entering meiotic prophase. It is approximately a 46.1kDa protein (384 amino acids).

The PRDM9-selective antibodies were generated against purified PR domain containing 9 protein. PRDM9-antibodies are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. FabGennix Int. Inc., will also provide limited quantities of antigenic blocking protein for PRDM9-901AP antibody. Antibodies to PRDM9 (PRDM9-901AP) will label ~46.1kDa protein in Western blot positive control samples for PRDM9 and several other tissues. FabGennix Inc. will also conjugate antibodies with secondary enzymes (alk-Pase or HRP) or fluorescent probes upon request at nominal cost.

For research use only, not for diagnostic or therapeutic use.