Synaptotagmin I
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| Syt1-101AP | Synaptotagmin I antibodies | Rabbit | AP | r, m, h, others | 100.0 ug | 150-500 ul |
$245.00
|
| FITC-Syt1 | FITC-conjugated Syt1 antibodies | Rabbit | FITC-AP | r, m, h, others | 100.0 ug | 150-500 ul |
$350.00
|
| P-Syt1 | Antigenic blocking peptide for Syt1 | n/a | Synthetic peptide | n/a | 250.0 ug | 100 ul |
$125.00
|
| PC-Syt1 | WB positive control for Syt1 | n/a | Protein in ready-to-use buffer | n/a | 0.0 ug | 150 ul |
$185.00
|
Synaptotagmin I (Syt1), located on human chromosome 12, is a member of synaptotagmin protein family. Syt1 is an integral membrane protein of synaptic vesicles and it is involved in both endocytosis and exocytosis (1). Syt1 is known to directly interact with the SNARE complex; it contains two C2 domains (C2A and C2B) that are homologous in sequence and 3-D in structure, but have distinct biochemical and biological properties (2). The C2A domain binds Ca2+ thus allowing Syt1 to act as a calcium sensor in the Ca2+-dependent release of neurotransmitter vesicles whereas the C2B domain binds inositol high polyphosphates (2). In vertebrates, Syt1 is required for a type of exocytosis (fast, synchronous release) (1). The intestinal epithelial Syt1 has been shown to play an important role in cAMP-stimulated endocytosis of apical membrane Na(+)-H(+) exchanger (3). Syt1 can also function as a protein receptor for Botulinum neurotoxins (BoNT/B and BoNT/G) (4).
The expression of Syt1 is negatively regulated by Akt (protein kinase B) and inhibition of Akt leads to an increase in the expression of Syt1 genes (5). A high expression of Syt1 is found in the brains of patients with refractory epilepsy (6). Deficiency of Syt1 in mice resulted in their death with 24 hours. These mice also exhibited motor dysfunction, reduction in body weight and growth (7). Syt1 is expressed throughout the brain, but it is mainly expressed in the cytoplasm and cytomembrane of neurons. Sty1 is approximately a 50.6kDa protein (422 amino acids).
The Synaptotagmin I (Syt1) antibodies were generated using peptide corresponding to human Syt1 protein. Syt1 antibodies are affinity purified over immobilized antigen based affinity chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. FabGennix Int. Inc. will provide limited quantities of antigenic blocking protein for competition assays involving Syt1 antibodies. Antibodies to Syt1 (Syt1-101AP) will label ~50.6kDa protein in Western blot positive control for Syt1 (PC-Syt1) and several other tissues. FabGennix Inc. will conjugate this and other antibodies from its catalog to either secondary enzymes (alk-Pase or HRP) or fluorescent probes at a nominal cost upon request. FabGennix also provides custom antibody production services for researchers that are looking for high affinity mono and polyclonal antibodies in various species. We specialize in making application specific antibodies that are useful in IHC, confocal and other applications where native antigen is detected.
For research use only, not for diagnostic or therapeutic use.
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