Suppressors of cytokine signaling (SOCS) proteins are intracellular regulators of receptor signal transduction, mainly Janus kinase/signal transducers and activators of transcription (JAK/STAT). The suppressor for cytokine signaling 3 is a key regulator of cytokine signaling in inflammatory diseases and in breast cancer progression. Over expression of SOCS3 in response to prolactin, suppresses STAT3 expression and abrogate STAT5 phosphorylation which resulted in decrease cell proliferation of T47D breast cancer cells. SOCS3 acts as an anti-proliferative agent in breast cancer cells whose mechanism of action and cross talk with other cellular pathways are complex (1). The IL6 signaling on both hepatic and hematopoietic cells is mediated through gp130 receptor and SOCS3 is a critical inhibitor of this pathway (2). SOCS3 knockout mice revealed significant enhancement in DNA replication and liver weight restoration after partial hepatectomy (PH) compared to littermate controls. The absence of SOCS3, STAT3 phosphorylation is prolonged activation of ERK1/2 is enhances after PH. SOCS protein inhibit cytokine signaling by targeting different component of signaling complex. These include either direct binding of SOCS to activated , tyrosine phosphorylated cytokine receptors ot JAK via their Src homology 2 doamin or via targeting receptor complexes for proteosomal degradation via SOC box (3). SOCS3 has been shown to regulate the activity of proline rich tyrosine kinase 2 (PYK2) in A549 cells as a result of epigenetic regulatory mechanism involving methylation of exon 2, and 5. Recent literature suggest that infections (hepatitis C and Leishmania donovani) caused an induction is SOCS3 that interfere with host immune responses.
There are eight SOCS family members known, SOCS1, SOCS2 and SOCS3, SOCS2 can enhance IL2 and IL3 induced STAT phosphorylation followed bt potentiate proliferation in response to cytokine stimulation. Expression of SOCS2 also resulted in marked proteosome-dependent reduction in SOCS3 and SOCS1 proteins. SOCS2 also bind to SOCS3 and elongin B/C to form an E3 ligase complex resulting in degradation of SOCS3 (4). SOCS3 is localized on chromosome 17q25 and was found to be expressed at higher concentration in skin of atopic dermatitis patients than in healthy individuals (5). SOCS3 is a 225 amino acid containing protein with apparent MW 28kDa. SOCS3 protein contains several phospho-tyrosine binding pockets at the N-terminal end, a SH2 superfamily domain and a SOCS-SOCS3 family domain near the C-terminal end called SOCS box.
The SOCS3-selective antibody was generated against a peptide from near N-terminus of the protein corresponding to amino acids 83-105 of the G6PC2 a 154 amino acid protein. The affinity purified mono epitope-specific rabbit polyclonal antibody strongly labels a broad band at 29 kDa inSOCS3 western blot control samples. The SOCS3 rabbit mono-epitope specific polyclonal antibodies are affinity purified on immobilized antigen affinity matrix, the affinity purified antibodies are also available in FITC and Biotin-conjugated form for use in IHC and cells labeling experiments. Limited quantities of antigenic blocking peptide and western blot positive controls samples are also available. FabGennix has a wide range of antibodies to innate immune and other targets, for a complete listing please visit www. FabGennix.com
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