The tumor protein D52 was found to be associated with immunoglobulin super-family receptor translocation-associated 1 (IRTA1) receptor in the b cells of normal lymphohematopietic tissue. The 28kDa protein TPD52 expression was also noted in only in normal and neoplastic epithelia. Unlike other B-cell associated molecules (Pax5, CD19, CD79a, CD20 and CD22) which are down regulated during differentiation form B-cell to plasma cells, the TPD52 expression reached maximum levcel at the plasma cell stage (1). TPD52 expression in thiel myeloma cell line was localized with Annexin IV in a calcium dependent manner suggesting that TPD52 and Annexin may regulate the secretory processes as has been observed in pancreatic acinar cells (1). The elevated expression of TPD52 was also found in lung and breast carcinomas where it was suggested to play a role in calcium mediated signal transduction and cell proliferation. The TPD52 was differentially expressed in fetal and adult tissues, in adult high expression levels were noted in brain, kidney, prostate, pancreas and intestine and at very low levels in lungs, liver, hematopoietic cells and gonads. During development the protein is expressed in epithelium of the intestine, stomach, olfactory and neuronal layers of retina (2). TPD52 expression was differential in Normal to low levels of this protein was found in The TPD52 protein represent a family of proteins with predicted coil-coil domain that demonstrate homo and heteromeric interactions with other tumor protein homologs hD52, hD53 and hD54 (3).
TPD52 was localized on chromosome 8p13 region. There are at least 3 isoforms reported in literature (TPD52 isoform 1, 2 and 3) with significant homology, the differences are in alternate splicing of exons. The tumor protein related family members are also implicated in cell proliferation, apoptosis and vesicle trafficking. The TPD52 is a 224 amino acid, 28 kDa protein (TPD52 isofrom 1). A testis specific tumor protein (hD55) was identified by yeast-two hybrids and GST pull-down assays which suggest that hD55 interacts with TPD52, hD53 and hD54 and itself. The hD55 is expressed 5-6 fold excess amount in adult testis than in fetal testis suggesting its role in spermatogensis (4).
The TPD52-selective antibodies were generated against synthetic peptides form unique regions of the TPD52 isoform 1. The TPD52 peptides were post-synthetically modified to achieve the desired antigenicity before injecting in to rabbits to obtained antibodies. The antibodies were isolated on an immobilized antigen based affinity matrix before stabilizing them in antibody stabilization buffer. Both these two antibodies label TPD52 protein as a single 28kDa band in PC-TPD52 samples. FabGennix Inc. provides Western blot positive controls for TPD52 in ready-to-use buffer for SDS-PAGE and western blotting applications. Limited quantities of antigenic blocking peptides for this antibody are also available (Inquire before placing orders). For a complete listing of all cancer related antibodies, visit our website at www.FabGennix.com.
For research use only, not for diagnostic or therapeutic use.
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Tumor protein D52
MSDS
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