Septin 3

Septin 3 (Sept3) antiboides.  Catalog # Sept-301AP, FITC-Sept3, P-Sept3, PC-Sept3. 

Accession # CAQ07552 and Alternate Nomenclature: SEP3; MGC133218; bK250D10.3.

Septin 3 (Sept3) belongs to the septin family of GTPases. GTPases are central regulators in signal transduction pathways in every aspect of cell biology (1). Septins bind to and hydrolyze GTP and most of these proteins polymerize to form filaments which may form cytoskeletal system that organizes higher-order structures (2). Septins were first discovered in yeast Saccharomcyes Cerevisiae, they are also found in animals and fungi but not in protozoa or plants (3). Septins are implicated in cytokinesis, exocytosis, tumorigenesis, apoptosis, synaptogenesis, neurodegeneration, and in functions involving changes in cell shape and in the specialized regions of the cell cortex (4). Humans have twelve septin genes, some of which generate multiple polypeptides either by alternative splicing or alternative translation start sites (2). The function of individual protein in septin family usually depends on its assembly into oligomeric complexes and highly ordered polymers (5).

Septin 3 interacts with sept5 and sept7 to form a heteromeric complex at nerve terminals (6). It is brain-specific protein abundant in synaptic terminals, but it is not required for normal neuronal development (7). It may play a secretory role in synaptic vesicle recycling. Septin 3 is phosphorylated by cGMP-dependent protein kinase I (PKG-I) (8). Its expression is upregulated by retinoic acid in human tetracarcinoma cell line (9). Septin 3 is located on human chromosome 22q13.2. It is expressed throughout the brain. Sept3 is approximately a 43kDa protein (358 amino acids).

The Sept3 antibodies were generated using peptide corresponding to human Sept3 protein. Sept3 antibodies are affinity purified over immobilized antigen based affinity chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. FabGennix Int. Inc. will provide limited quantities of antigenic blocking protein for competition assays involving Sept3 antibodies. Antibodies to Sept3 (Sept-301AP) will label ~43kDa protein in Western blot positive control for Sept3 (PC-Sept3) and several other tissues.  FabGennix Inc. will conjugate this and other antibodies from its catalog to either secondary enzymes (alk-Pase or HRP) or fluorescent probes at a nominal cost upon request.  FabGennix also provides custom antibody production services for researchers that are looking for high affinity mono and polyclonal antibodies in various species. We specialize in making application specific antibodies that are useful in IHC, confocal and other applications where native antigen is detected. For a complete listing of all FabGennix antibodies please visit www.Fabgennix.com. 

For research use only, not for diagnostic or therapeutic use.