Oxidoreductase Type 4
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| Wox-401AP | Oxidoreductase 4 antibodies | Rabbit | AP | r, m, h | 100.0 ug | 150-500 ul |
$245.00
|
| FITC-Wox4 | FITC-conjugated antibodies | Rabbit | FITC-AP | r, m, h | 100.0 ug | 150-500 ul |
$350.00
|
| P-Wox4 | Antigenic blocking peptide | n/a | Synthetic peptide | n/a | 250.0 ug | 100 ul |
$125.00
|
| PC-Wox4 | WB positive control for Wox4 | n/a | Protein in ready-to-use buffer | n/a | 0.0 ug | 150 ul |
$185.00
|
The WWOX (WW-domain containing oxidoreductase) is a candidate tumour suppressor gene spanning the same chromosome region, 16q23, as the second most common fragile site (FS), FRA16D (1). Several deletion mutants of this protein have been identified using comparative genomic hybridization (CGH). Loss of DNA copy-number confined to band 16q23 was detected by CGH in several cell lines. Although homozygous deletions of the WWOX gene were not detected, WWOX mRNA expression was absent or lower in 60% of cell lines (1). A number od Wwox proteins have been identified ranging from 47kDa to 8 kDa (Wwxo-1 thru Wwox-9). It has been shown that expression of a novel WOX (WUSCHEL related homeobox) gene family members reveal early embryonic patterning events in Arabidopsis. WOX2 and WOX8 are co-expressed in the egg cell and zygote and become confined to the apical and basal daughter cells of the zygote, respectively, by its asymmetric division. Expression of WOX5 shows that identity of the quiescent center is initiated very early in the hypophyseal cell, and highlights molecular and developmental similarities between the stem cell niches of root and shoot meristems. WOX9 expression is initiated in the basal daughter cell of the zygote and subsequently shifts into the descendants of the apical daughter apparently in response to signaling from the embryo proper.
It has been shown that Wwox 1 and its tyrsine 33 phosphorylated form is Down-regulated in the neurons of AD patients hippocampi compared to aged matched controls (2). In vitro down-regulation of Wwox 1 by SiRNA remarkedly heperphosphorylate Tau proteins at position of WW domain-containing oxidoreductase (Wox 1) induces Tau phosphorylation at position Thr 212/231 and Ser 515/516, enhanced phophosrylation of Glycogen synthase kinase 3 beta, and ERK (2). The interaction of WOX1 with Tau via its short-chain alcohol dehydrogenase/reductase domain is likely to play a critical role in regulating Tau hyperphosphorylation and neurofibrillary tangles (NFTs) in the neurons of Alzheimer’s disease (AD).
WWOX1 encodes a 46 kDa protein that contains two WW domains and a short-chain oxidoreductase (SDR) domain. The Wox 1-selective antibodies were generated in rabbits against unique N-terminal peptide that are common to Wox1 and Wox 4 proteins. Wox-1 antibodies also labels Wwox 4 protein but does not cross reacts to other Wwox proteins. FabGennix Inc. has generated specific mono-epitope-specific rabbit anti-Wwox1 antibodies using linear and multiple antigenic peptide (MAP) methodology. These antibodies have been characterized by ELISA, Westerns and dot blot assays using various cell lines. FabGennix Inc. has also produced other elated antibodies that are listed under “Antibdoies to Diagnostic markers” that will facilitate research on cancer detection. Anti-Wwox 1 antibodies are availabel in affinity purified form and can be labeled with fluorophores and secondary enzymes upon request. FabGennix Inc. also provides limited quantities of antigenic blocking peptides for Wwox 1antibodies.
For research use only, not for diagnostic or therapeutic use.
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