Estrogen Receptor beta

Estrogen, a steroid hormone, is a key regulator of growth and differentiation in a broad range of target tissues and, it is also implicated in breast and endometrial cancer and osteoporosis (1). Like all steroid hormones, estrogen readily diffuses across cell membrane and binds to and activates estrogen receptors (α and β) which then up-regulate the expression of many genes. These receptors belong to the superfamily of nuclear receptors, more specifically to the family of steroid receptors that act as ligand-regulated transcription factors (2). Estrogen binding receptor protein was first discovered in the early sixties, followed by cloning of this receptor in the mid-eighties. In 996, this receptor was renamed estrogen receptor α (ERα) due to cloning of an additional estrogen receptor from rat prostrate. This novel receptor was designated estrogen receptor β (ERβ) (3). ERβ is located on mouse chromosome 12 D1-D3. Recent reports have shown that ERβ is important for the prevention of age-related hearing loss, proper cAMP signaling pathway, polyovular follicles in mouse ovaries and also important in regulating tyrosine hydroxylase expression in the moue locus coeruleus (4).

Any disruption in estrogen receptor β signaling has been shown to cause negative effects on ovulation and fertility (4). ERβ deficiency in mice was found to be associated with increase in small intestine tumorigenesis. ERβ deficiency also leads to increase epithelial proliferation, decreased apoptosis, and accumulation of incompletely differentiated cells in mouse ventral prostrates (5). In male mice, ERβ deletion affected the structure of their arteries. ERβ is expressed in kidney, spleen, testis and skeletal muscles. It is approximately a 72kDa protein (567 amino acids).

The Estrb1-selective antibodies were generated against purified Estrogen receptor beta isoform 1 protein. Estrb-antibodies are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. FabGennix Int. Inc., will also provide limited quantities of antigenic blocking protein for Estrb-101AP antibody. Antibodies to Estrb-1 (Estrb1-101AP) will label ~72kDa protein in Western blot positive control samples for Estrb-1 and several other tissues. FabGennix Inc. will also conjugate antibodies with secondary enzymes (alk-Pase or HRP) or fluorescent probes upon request at nominal cost.

For research use only, not for diagnostic or therapeutic use.