Runt-related Transcription factor 1

Accession #/Synonyms: AAI36381; RUNX1 protein, Acute myloid leukemiaprotein 1/AML1,PEA2-alpha; CBF-alpha2;SL3/AKV core-binding factor B subunit; PEBP2 .  

Runt-related Transcription factor1 (Runx1) belongs to the family of Runt-related  to a transcription factors comprising of 3  subtypes (Runx1-3).  Regulation of cell proliferation by RUNX proteins represents an evolutionarily conserved activity.  All vertibrates ave three Runx genes and all three genes have P1 and P2 promotors. The Runx3 P1 and P2 promoter regions showed that a small (around 600 bp) P1 element could direct cell-type-specific expression with fidelity (1).   Runx1- transcription factor is an essential regulator of hematopoiesis and has multiple isofroms arising from differential splicing and utilization of two promotors. At least three variants have been characterized for Runx1 (Runx1a, b and c).  all Runx transcription factors (Runx1, Run2 and Runx3) bind to the same DNA motif, hence their  temporal and spatial expression is during development is tightly regulated.  The c isoform is transcribed from the use of distal promotor that resulted in a transcript that have 32 amino acids unique to this isoform (2)1).  The Runx1b has 5 unique N-terminal amino acids and originated form the use of proximal promotor P2.  Runx1 is a powerful regulator of hematopoiesis.   Runx1 transcription factor is highly expressed at a CD4/CD8-double-negative (DN) stage of thymocyte development but is down-regulated when cells proceed to the double-positive (DP) stage (3).

Runx1 function can be regulated by post-translational modifications (arginine methylation by PRMT1) and protein–protein interactions.  The PRMT1 methylation of R206 and R210 on Runx1 abrogated its association with corepressor SIN3A (4).  Runx1 mutation has been  seen in patients with familial platelet disorders, thrombocytopenia.  RUNX1 also directly regulates G1 to S cell cycle progression of the hematopoietic cells, which is accounted for by the phosphorylation of  S303 by cdks lead to ubiquitin-mediated degradation during G2/M phase (5).  Runx1 is a 58kDa protein (480aa).  There are several isoforms that are expressed in hematopoietic cells.

Runx1- selective antibodies were generated against a peptide taken form N-terminal region spanning 1-75 amino acids. The 23mer peptide was conserved in Runx1 isoform 1, 2 and 3 of human Runx1.  The antibodies do not cross react to onther Runx subtypes. The anti-Runx1-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions.  The Runx1antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage.  FabGennix Int. Inc. provides Runx1Western blot positive control samples in “ready-to-use” SDS-PAGE sample buffer.  Limited quantities of antigenic blocking peptide for Runx1antibody is also available, please inquire before ordering.  FabGennix Int. Inc., will also conjugate this antibody to fluorophores and other secondary enzymes at a nominal price. FabGennix Int. Inc., provides custom antibody production services for researchers that are looking for high affinity mono and polyclonal antibodies in various species. We specialize in making application specific antibodies that are useful in IHC, confocal and other applications where native antigen is detected. For a complete listing of all FabGennix antibodies please visit www.Fabgennix.com. 

For research use only, not for diagnostic or therapeutic use.