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Myeloperoxidase

Catalog# Product Description Host Species Nature Cross reactivity Quantity Volume Price
MPO-101AP Myeloperoxidase N-terminal antibodies Rabbit AP r, m, h 100.0 ug 150-500 ul $245.00
MPO-112AP Myeloperoxidase mid-region antibodies Rabbit AP r, m, h 100.0 ug 150-500 ul $245.00
FITC-MPO100 FITC-conjugated N-terminal antibodies Rabbit FITC-AP r, m, h 100.0 ug 150-500 ul $350.00
FITC-MPO110 FITC-conjugated mid-region antibodies Rabbit FITC-AP r, m, h 100.0 ug 150-500 ul $350.00
P-MPO100 N-terminal antigenic blocking peptide n/a Synthetic peptide n/a 250.0 ug 100 ul $125.00
P-MPO110 Mid-region antigenic blocking peptide n/a Synthetic peptide n/a 250.0 ug 100 ul $125.00
PC-MPO WB positive control for MPO n/a Protein in ready-to-use buffer n/a 0.0 ug 150 ul $185.00
Myeloperoxidase (MPO) is a functionally important component of the normal human neutrophil azurophil granule of polymorphonuclear leukocytes, host defense system.  MPO is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. The inflammatory component of allergic reactions can be measured by eosinophil cationic protein (ECP, specific for eosinophils) and myeloperoxidase (MPO, specific for neutrophils) measurement (1). Neutrophils, especially in acute infection or the myeloid leukemias, may shed platelet-sized particles that can readily be distinguished from true platelets because they contain neutrophil myeloperoxidase, an enzyme that is not inhibited by glutraldehyde. Traditionally Myeloperoxidase was considered as a serological marker for ulomatosis (Churg-Strauss syndrome), the main target of anti-neutrophil cytoplasm antibodies (ANCA), Low to moderate anti-Myeloperoxidase autoantibody levels are also reported in rheumatoid arthritis. Recently it was shown that MPO participates in the initiation and progression of cardiovascular disease. It possesses potent pro-inflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is uncertain systemic vasculities e.g. periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic grander consideration as one of the most promising cardiac markers. 
Human myeloperoxidase, a 745 aa protein is produced by a gene composed of 12 exons and 11 introns.The sequence was found to contain an open reading frame, 2,235 nucleotides coding for a protein of 745 amino acids with a calculated Mr of 83,868. The heavy chain of myeloperoxidase, consisting of 467 amino acids, was located on the COOH terminus half of the protein. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59 - 64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW 150 kDa and theoretical pI 9.2, each of which is associated with a heme-like prosthetic group. The heme environment, defined by X-ray crystallographic analyses of MPO appears to be highly conserved in four mammalian peroxidases (lactoperoxidase, thyroid, esinophil and Myeloperoxidase). MPO when heated under non-reducing, denaturing conditions, MPO is cleaved to produced 22 and a 38kDa proteins, these two species are produced via a novel autolytic cleavage at Meth409 and Pro410 (2).
FabGennix International Inc. has produced affinity purified antibodies to Myeloperoxidase protein. These antibodies are generated in rabbit using synthetic peptides corresponding to MPO proteins. The Myeloperoxidase antibodies are then affinity purified using immobilized antigenic affinity chromatography. Limited quantities of blocking antigenic peptides are also available (please inquire).   FabGennix, Inc., will also provide Western blot positive controls for most of these antibodies in ready-to-use buffer for easy identification of respective proteins. FabGennix International Inc. will label MPO antibodies to fluorophores or to alkaline-phoshpatase or horseraddish peroxidase for a nominal price. FabGennix International Inc., has produced many diagnostic antibodies, for a complete listing please visit www.FabGennix.com.

For research use only, not for diagnostic or therapeutic use.

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Myeloperoxidase

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