Septin 7

Accession # AAB17294 and Alternate Nomenclature: CDC3; CDC10; SEPT7A; Nbla02942; SEPT7

Septin 7 (Sept7) belongs to the septin family of GTPases. GTPases are central regulators in signal transduction pathways in every aspect of cell biology (1). Septins bind to and hydrolyze GTP and most of these proteins polymerize to form filaments which may form cytoskeletal system that organizes higher-order structures (2). Septins were first discovered in yeast Saccharomcyes Cerevisiae, they are also found in animals and fungi but not in protozoa or plants (3). Septins are implicated in cytokinesis, exocytosis, tumorigenesis, apoptosis, synaptogenesis, neurodegeneration, and in functions involving changes in cell shape and in the specialized regions of the cell cortex (4). Humans have twelve septin genes, some of which generate multiple polypeptides either by alternative splicing or alternative translation start sites (2). The function of individual protein in septin family usually depends on its assembly into oligomeric complexes and highly ordered polymers (5).

Septin 7 interacts with septin 6 to form filaments (6). It also interacts with sept9 and sept11 (7). Sept7 is essential for the process of mitosis and normal organization of actin cytoskeleton (8). Sept7 filaments distribute along the mitotic spindle and terminate at the kinetochore marked by centromere-associated protein E (CENP-E). It is required for a stable association of CENP-E with kinetochore (9). Suppression of this protein causes a reduction in the tension at kientochores, thus septin 7 also plays a key role in distributing CENP-E to the kinetochore and in chromosome alignment (9). Sept7 is located on human chromosome 7p14.2. It is expressed ubiquitously. Sept7 is approximately a 50kDa protein (417 amino acids).

The Sept7 antibodies were generated using peptide corresponding to human Sept7 protein. Sept7 antibodies are affinity purified over immobilized antigen based affinity chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. FabGennix Int. Inc. will provide limited quantities of antigenic blocking protein for competition assays involving Sept7 antibodies. Antibodies to Sept7 (Sept-701AP) will label ~50kDa protein in Western blot positive control for Sept7 (PC-Sept7) and several other tissues.  FabGennix Inc. will conjugate this and other antibodies from its catalog to either secondary enzymes (alk-Pase or HRP) or fluorescent probes at a nominal cost upon request.  FabGennix also provides custom antibody production services for researchers that are looking for high affinity mono and polyclonal antibodies in various species. We specialize in making application specific antibodies that are useful in IHC, confocal and other applications where native antigen is detected. For a complete listing of all FabGennix antibodies please visit http://fabgennix.com

For research use only, not for diagnostic or therapeutic use.