GTP cyclohydrolase I
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| GTPCHI-101AP | GTPCHI affinity purified N-terminal antibodies | Rabbit | Affinity purified antibody | r, m, h | 100.0 ug | 150-500ul |
$245.00
|
| GTPCHI-112AP | GTPCHI affinity purified C-terminal antibodies | Rabbit | Affinity purified antibody | r, m, h | 100.0 ug | 150-500ul |
$245.00
|
| FITC-GTPCHI | FITC-conjugated antibody | Rabbit | FITC-conjugated antibodies | r, m, h | 100.0 ug | 150-500ul |
$350.00
|
| FITC-GTPCHI2 | FITC-conjugated C-terminal antibody | Rabbit | FITC-conjugated antibodies | r, m, h | 100.0 ug | 150-500ul |
$350.00
|
| P-GTPCHI1 | Antigenic blocking N-peptide | n/a | Synthetic peptide | n/a | 250.0 ug | 100ul |
$125.00
|
| P-GTPCHI2 | Antigenic blocking C-peptide | n/a | Synthetic peptide | n/a | 250.0 ug | 100ul |
$125.00
|
| PC-GTPCHI | WB positive control for GTPCHI | n/a | Protein in ready-to-use bufer | n/a | 0.0 ug | 150ul |
$185.00
|
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uanosine triphosphate cyclohydrolase I (GTPCHI) is the rate limiting enzyme for the synthesis of tetrahydrobioptrin, a cofactor for hydroxylation reaction in several pathways. GTPCHI is a critical enzyme in catecholamine function and is rate limiting for the synthesis of the catecholamine co-factor tetrahydrobiopterin (6BH)4, a key cofactor necessary for nitric oxide synthase and for the hydroxylases that are involved in the production of catecholamines and serotonin. (6R)-L-Erythro 5,6,7,8 tetrahydrobiopterin (6BH(4)) is crucial in the hydroxylation of L-phenylalanine-, L-tyrosine-, and L-tryptophan-regulating catecholamine and serotonin synthesis as well as tyrosinase in melanogenesis. The GTPCHI feed back regulatory protein (GFRP) binds to GTPCHI in presence of phenylalanine and inhibit its activity in the presence of biopterin. The shear stress in vasculature caused an up-regulation of eNOS and GTPCHI accompanied by increased blood flow. The GTPCHI increase caused a rise in BH4 production which required for sustained vasorelaxant response that increases the blood flow. The cofactor supply of (6BH)4 is indeed controlled by GFRP and GTPCHI in epidermal keratinocyltes and melanocytes for the biosynthesis of catecholamine and serotonin.
GTPCHI is a 241 aa (30 kDa) cytosolic protein that interact with GFPR. The expression of GTPCHI is reduced in nigrostraital neurons in middle and aged rats, monkeys and humans. The age related decrease in GTPHCI nigral neurons suggest a phenotypic-down regulation of this enzyme rather than generalized necrosis of cells and may be responsible for the generalized age-related decrease in dopaminergic tome with nigrostriatal system (1). Gene therapy approach for Parkinsonian condition was tested using purified adeno-associated virus containing tyrosine hydroxylase (TH) and GTPCHI to infect 6-OH denervated rat striatum. The viral delivery system indeed showed >90% of viral infected neurons to be TH positive (2), however, the production of L-DOPA was only evident in transfections with TH nd GTPCHI, suggesting that the trans-gene expression of TH and GTPCHI can be used to successfully deliver L-DOPA to striatum (2). The activity of GTPCHI is reduced by 50% in CNS of rats exhibiting chronic hepatic encephalopathy (3).
The GTPCHI-selective antibodies were generated against 2 peptides form unique regions on the GTPHCI protein. FabGennix Inc. has generated epitope specific rabbit anti-GTPCHI polyclonal (epitope-specific) antibodies utilizing linear and cyclic peptide sequences. The GTPCHI antibodies are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulin are stabilized in antibody stabilization buffer. Limited quantities of the antigenic blocking peptide for GTPCHI antibodies are also available (inquire for availability). FabGennix Inc. will also conjugate antibodies with enzymes or fluorescent probes as custom service upon request at a reasonable cost.
For research use only, not for diagnostic or therapeutic use.
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