Aquarius
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| AQR-101AP | Aquarius antibodies | Rabbit | AP | m | 100.0 ug | 150-500 ul |
$245.00
|
| FITC-AQR | FITC-conjugated antibodies | Rabbit | FITC-AP | m | 100.0 ug | 150-500 ul |
$350.00
|
| P-AQR | Antigenic blocking peptide | n/a | Synthetic peptide | n/a | 250.0 ug | 100 ul |
$125.00
|
| PC-AQR | WB positvie control for AQR | n/a | Protein in ready-to-use buffer | n/a | 0.0 ug | 150 ul |
$185.00
|
The gene trap screen methodology was used to identify and characterize a new and novel gene from retinoic acid dependent morphologically differentiated mouse embryonic stem cells, called Aquarious (AQR). The promoterless lacZ marker was used to trap the genomic locus and to determine the expression pattern of the gene. AQR transcripts are strongly induced in response to RA in vitro in mouse embryonic stem cells (1). The expression of AQR during embryogenesis was localized in the mesoderm and neuronal crest region and its target tissue and in neuroepithelium. Expression was first detected at 8.5 days postcoitum, when neural crest cells are visible at the lateral ridges of the neural plate.
The gene-trapped AQR locus was transmitted through the mouse germ line in three genetic backgrounds. The AQR gene ratio in the F2 generation was 1:2:1 as predicted by the mendalion distribution and was observed in all the backgrounds suggesting that homozygous mice are viable. The homozygous mice are normal in weight and size and breed normally. The gene trap insertion, however, does not seem to generate a null mutation, because AQR transcripts are still present in the homozygous mutant animals. The AQR open reading frame has weak homology to RNA-dependent RNA polymerases (RRPs) of the murine hepatitis viruses and contains an RRP motif. AQR was mapped to mouse chromosome 2 between regions E5 through F2 by using fluorescence in situ hybridization analysis. (1).
The AQR-selective antibodies were generated against synthetic peptide corresponding to residues 273-291 of mouse embryonic stem cell AQR kinase. The antibodies to AQR are affinity purified over immobilized antigen matrix based chromatography. The purified antibodies are stabilized in antibody stabilization buffer containing preservatives. FabGennix Int. Inc., will also provide Western blot positive controls for Aquorious protein in ready-to-use buffer for SDS-PAGE and Western blot analyses. These positive controls can be used as internal standards for quantitaive western blotting purposes. FabGennix International Inc. also carries limited quantities of antigenic blocking peptide for AQR antibodies. Please inquire about pricing and availability of these products. Other related antibodies that are carried by FabGennix Int. Inc. include vitamin A transporter Strah6, p21 activated protein kinase (p65pak), ACK1, Anti-protein synthesis initiation factor 4 (eIF4E), Anti-protein phosphatase 2A inhibitor protein. FabGennix Inc. will also conjugate antibodies with fluorescent probes upon request at a reasonable cost. A complete list of these and other items can be viewed at www.FabGennix.com.
For research use only, not for diagnostic or therapeutic use.
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