Cannabinoid Rec 2

Accession/Synonym: P47936; Cannabinoid receptor 2; CB-2; hCB2; CX5.
Endocannabinoids (eCBs) are endogenous lipid mediators capable of binding to and functionally activating G-protein coupled cannabinoid receptors (CB₁ and CB₂) to modulate synaptic function.  Both CB₁ and CB₂ receptors belong to the superfamily of  7TMD containing G protein-coupled receptors (GPCRs) and act mainly through inhibitory G proteins (G_i/o). Activation of CB1/CB2 led to a decrease in cellular cAMP levels. Activation of CB1/CB2 can activate post receptor signaling pathways mediated by different kinases activating nuclear factor-kappaB (NF-kappaB), nuclear factor of activated T cells (NFAT) and activator protein-1 (AP-1) DNA binding. Binding of agonist to CB1/CB2 receptors led to epigenetic regulation of several genes. CB1/Cb32 receptors also exhibited anti-nociceptive action when cannabinoids are injected directly into the hind paw skin of uninjured rats.. 

The cannabinoid receptors CB1/CB2 are expressed in CNS and in peripheral tissues including cells of hematopoietic origin, expressed in skin in suprabasal and hair follicles, in brain by neurons and glial cells and by osteoblasts, osteocytesm osteoclasts.  Both receptors are localized in nerve fibers of keratinocytes, dorsal root ganglion tissue, and in other peripheral tissues. CB2 is expressed predominantly outside the CNS and is markedly up-regulated after spinal nerve ligation.  The cannabinoid delta-9-tetrahydrocannabinol (THC) binds to CB1/CB2 receptors and produce psychodelusional effects.  The amino acid sequence homology between CB1 and CB2 is unusually low for GPCR that bind the same neurotransmitter/neuromodulator ligand. The role of CB2 receptor in CNS in addiction and major depression is ambiguous and controversial (1).  The cannabinoid system is immunomodulatory and has been targeted as a treatment for the central nervous system (CNS) autoimmune disease multiple sclerosis (2). CB2 receptor is located on chromosome and has apparent MW of 42kDa comprising of 347aa. 

The CB2R antibodies were generated using peptide corresponding to mouse CB2R protein. The CB2R antibodies are affinity purified over immobilized antigen based affinity chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. FabGennix Int. Inc. will provide limited quantities of antigenic blocking peptide/protein for competition assays involving CB2R antibodies. Antibodies to CB2R (CB2R-201AP) will label ~45kDa protein in Western blot positive control for CB2R (PC-CB2R) and several other cells and tissues. FabGennix Inc. will conjugate this and other antibodies from its catalog to secondary enzymes (alk-Pase or HRP) or fluorescent probes at a nominal cost upon request.  FabGennix also provides custom antibody production services for researchers who are looking for high affinity mono and polyclonal antibodies in various hosts. We specialize in making application specific antibodies that are useful in IHC, confocal and other applications where antigen in native conformation is detected. For a complete listing of all FabGennix antibodies please visit www.Fabgennix.com. 

Immunogen:            Synthetic peptide unique to CB2 receptor and common in both CB2L and CB2S type receptors.  The epeptide reside between amino acids 300-347.  The peptide was covalently modified to obtained desired antigenicity before conjugating to a carrier protein.   

Concentration:         CB2R-201AP and CB2R-FITC:  IgG concentration 0.61-0.63mg/ml in antibody stabilization buffer.

Applications:           Antibody CB2R-201AP is ideal for WB and ELISA applications, other applications have not been tested. The species cross reactivity for this antibody has not been fully elucidated.  The dilution for this antibody is for reference purposes only, investigators are expected to determine the optimal conditions for a specific assay.  WB: > 1:500; IMM & i.p pull-down assays: n.d; IHC n.d.  ELISA <1:10,000.  Application of this antibody in protocols not listed here does not necessarily exclude its use in such procedures.  Investigators using this antibody in applications not listed here can request for a complimentary sample of this antibody.

Reactivity:               This antibody detects a band of approximately ~45kDa in PC-CB2R samples.

Protocols:                Standard protocol for various applications (WB; IMM and IHC) of this antibody is provided with the product specification sheet; however, FabGennix Inc. strongly recommends investigators to optimize conditions for use of this antibody in their laboratories.

 

For research use only, not for diagnostic or therapeutic use.