Disemboided

n Fruit Fly (Drosophila melanogaster) steroid hormones play an important role in the reproduction, development and homeostasis between transitions between various stages of development (1).  In D melonogaster the hormone ecdysone (E) is converted to  20-hydroxyacdysone by  a P450 enzyme in peripheral target tissue.  The genomic and regulatory effect of this hormone are mediated by activation of a nuclear hormone receptor an activation of transcriptional cascade (2).  Recently it has been shown that a product of the Disemboided locus as being potentially involved in the production control of ecdysteroid as a member of the cytochrme P450 family monooxygenese (CYP302A). Mutation in the Disemboided protein result in a number of distinct phenotypes in D. melonogaster including disruption in dorsal closure, head evolution, gut morphogenesis, and failure to produce embryonic cuticle.   Most of these disruptions are the result of low affinity of 20E to its receptor due to significant reduction in the expression of IMP-E1 gene.  Several other mutations in the “Howloween” group of proteins, including Shadow (sad) also resulted in similar phenotypes (3, 4).  The co-expression of dib and sad causes increased metabolism of ketotriol to ecdyson (2) and the two proteins are concentrated with in the individual segment (2). 

The Anti-disemboided-selective antibodies were generated against conserved sequences near the N-terminus and the C-terminal end of the protein that are unique to Disemboided (dib) protein.  The Disemboided-selective antibodies label dib proteins in fruit fly extracts.  The Disemboided antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded epitope-specific antibodies.  The Disemboided antibodies label a 58-60kDa protein in D. melanogaster extracts.  Anti-Shadow-selective antibodies are also available in affinity-purified form for confocal, Western blotting and immunocytochemical analyses.  FabGennix Inc. will also conjugate antibodies with fluorescent probes upon request at extra charge. 

FabGennix Inc. also provides several antibodies against proteins that are involved Drosophila melanogaster research such as several Anti-PDE antibodies, Anti-Wishfulthinking, Anti-Baboon, Anti-Shade, Anti-Saxophone, Anti-SARA anchor protein, Anti-Phantom and Anti-Punt protein.  FabGennix Inc employs cyclic peptide methodology for generating antibodies, which results in higher titer and specificity (6).  FabGennix, Inc., will also provide Western blot positive controls for most of these antibodies in ready-to-use buffer for easy identification of respective proteins.  Limited quantities of antigens are also available.  Please enquire for their availability before ordering.

For research use only, not for diagnostic or therapeutic use.