Drosophila Melanogaster TGFbeta pathway proteins
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| Medea-101AP | Medea antibodies | Rabbit | Affinity purified | Fruit Fly | 100.0 ug | 150-500 ul |
$245.00
|
| FITC-Medea | FITC-conjugated Medea antibodies | Rabbit | FITC-conjugated antibodies | Fruit Fly | 100.0 ug | 150-500 ul |
$350.00
|
| P-Medea | Antigenic blocking peptide | n/a | Synthetic peptide | n/a | 250.0 ug | 100 ul |
$125.00
|
| PC-Medea | WB positive control for Medea antibodies | n/a | Protein in ready-to-use buffer | n/a | 0.0 ug | 150 ul |
$185.00
|
The fly Drosophila melanogaster is one of the most intensively studied organisms in biology and serves as a model system for the investigation of many developmental and cellular processes common to higher eukaryotes, including humans. Approximately 120 mb euchromatic portion of the fly genome ha been sequenced using a shotgun sequencing approach. The entire fly genome encodes for approximately 13,600 genes with vast functional diversity. The dorsal ventral patterning in vertebrate embryos is regulated by members of TGF-beta family of growth and differentiation factors. The receptors for ligands in theTGF-beta superfamily are kinases but not much is known about their down stream components. In drosophila the decapentaplegic protein (dpp), a homolog of vertebrate BMP2 and BMP4 is involved in dorsal ventral axial patterning. Several downstream component of dpp signaling are characterized including MAD and MEDEA that codes a unique predicted cytoplasmic with no identifiable functional domain. Phenotypic analysis of the new Medea mutations indicates that Medea, like Mad, is required for both embryonic and imaginal disc patterning. Complete elimination of maternal and zygotic Medea activity in the early embryo results in a ventralized phenotype identical to that of null dpp mutants, indicating that Medea is required for all dpp-dependent signaling in embryonic dorsal-ventral patterning (1).
Two Smads have been identified in Drosophila. Mothers against dpp (Mad) is a pathway-specific Smad, whereas Daughters against dpp (Dad) is an inhibitory Smad genetically shown to antagonize Dpp signaling.
The protein coded by Medea has a striking sequence similarities to human SMAD4. Medea (SMAD4)AD forms heteromeric complexes with Drosophila SMAD4 (Medea) upon phosphorylation by thickvein (Tkv), a type 1 receptor for Dpp (2). Like dpp, Medea is essential for embryonic dorsal/ventral patterning. However, Mad is essential in the germline for oogenesis whereas Medea is dispensable (3). MEDEA is localized in the cytoplasm, is not regulated by phosphorylation, and requires physical association with MAD for nuclear translocation. The Medea protein is a 745 amino acid (MW 87-89kDa) cytosolic protein expressed during early developmental stages of fly. The mammalian counter parts of Medea is SMAD4 acting as a down-stream transcriptional activators. SMAD2 and SMAD3 are phosphorylated as a result of the canonical cascade through ligand binding and receptor kinase activation. These phosphorylated SMADs (pSMAD) associate with SMAD4, a co-SMAD, and transcriptionally activate TGFbeta-mediated genes. In drosophila Medea is not phosphorylatedon during either development or by its physiological ations.
The Medea-selective antibodies were generated against synthetic peptides form unique regions on the C-terminal end of protein. The Mad peptide was post-synthetically modified to achieve the desired antigenicity before injecting in to rabbits to obtained antibodies. The antibodies were isolated on an immobilized antigen based affinity matrix before stabilizing them in antibody stabilization buffer. The Medea antibodies label Medea A protein at 89kDa band in PC-Medea samples. There are at least 2 more bands found in fly extracts. FabGennix Inc. provides Western blot positive control samples for Medea in ready-to-use buffer for SDS-PAGE and western blotting applications. Medea positive control appears as a diffuse band at 89kDa. FabGennix International Inc., has also produced antibodies to fruit fly TGFbeta pathway targets, for a complete listing please visit www.FabGennix.com. Limited quantities of antigenic blocking peptide for Medea antibody is also available (Inquire before placing orders). For a complete listing of all Drosophila related antibodies, visit our website at www.FabGennix.com. FabGennix International Inc., will conjugate and couple these antibodies to fluorescent probes and secondary enzymes at a nominal price.
For research use only, not for diagnostic or therapeutic use.
Data Sheets
MSDS
General Information
Citations
Search FabGennix Product Citation Data Base
Search