MutS1 human homolog
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| MSH4-401AP | MSH4 N-terminal antibodies | Rabbit | AP | A. thaliana (yeast) | 100.0 ug | 150-500 ul |
$245.00
|
| MSH4-412AP | MSH4 mid-region antibodies | Rabbit | AP | A. thaliana (yeast) | 100.0 ug | 150-500 ul |
$245.00
|
| FITC-MSH4-100 | FITC-conjugated N-terminal antibodies | Rabbit | FITC-AP | A. thaliana (yeast) | 100.0 ug | 150-500 ul |
$350.00
|
| FITC-MSH4-110 | FITC-conjugated mid-region antibodies | Rabbit | FITC-AP | A. thaliana (yeast) | 100.0 ug | 150-500 ul |
$350.00
|
| P-MSH4-100 | N-terminal antigenic blocking peptide | n/a | Synthetic peptide | n/a | 250.0 ug | 100 ul |
$125.00
|
| P-MSH4-110 | Mid-region antigenic blocking peptide | n/a | Synthetic peptide | n/a | 250.0 ug | 100 ul |
$125.00
|
| PC-MSH4 | WB positive control for MSH4 | n/a | Protein in ready-to-use buffer | n/a | 0.0 ug | 150 ul |
$185.00
|
Six strong homologs of the bacterial MutS DNA mismatch repair (MMR) gene have been identified in the yeast Saccharomyces cerevisiae. With the exception of the MSH1 gene, the involvement of each homolog in DNA repair and recombination during meiosis has been determined previously. Five of the homologs have been demonstrated to act in meiotic DNA repair (MSH2, MSH3, MSH6 and MSH4) and/or meiotic recombination (MSH4 and MSH5). Several strong homologs of bacterial MutS DNA mismatch repair (MMR) genes have been identified (MSH1-MSH6), with the exception of the MSH1 gene, all others have significant role in recombination and DNA repair during meiosis.
MSH4, a meiosis-specific member of the MutS-homolog family of genes, is required for normal levels of recombination and fertility in budding yeast, mouse, and Caenorhabditis elegans. The arabipdosis specific homolog of MSH4 (AtMSH4) in reproduction was characterized in floral tissues (1). The expression of MSH4 in A thaliana is limited to early meiotic phase I preceding the synapsis of homologous chromosomes. The insertion of AT-DNA mutant (ATmsh4) exhibited normal vegetative growth but significant reduction in fertility consistent with meiotic defect which was further confirmed by cytological staining of meiosis. Similar reduction in meiosis was observed with RNAi dependent down regulation of MSH4 which is the result of delayed or incomplete prophase I chromosome sysnapsis and metaphase chiasma frequency is greatly reduced compared to wild type leading to univalence and nondisjunction in budding yeast (1).
MSH4 protein has several function domains in cluding MutS domain III which is found in MutS family of DNA mismatch repair protein and is found associated with pfam00488, pfam05188, pfam01624 and pfam05190; pfam05192. The MutS1 homolog in eukaryotes. The MutS protein initiates DNA mismatch repair by recognizing mispaired and unpaired bases embedded in duplex DNA and activating endo- and exonucleases to remove the mismatch. A thaliana MSH4 protein is a 792 amino acid (98-100kDa) protein expressed din nuclear compartment.
The MSH4-selective antibodies were generated against 2 synthetic peptides corresponding to residues 40-57 and 774-792 of Arabidopsis thaliana MSH4 protien. The antibodies to MSH4 are affinity purified over immobilized antigen matrix based chromatography. The purified antibodies are stabilized in antibody stabilization buffer containing preservatives. FabGennix Int. Inc., will also provide Western blot positive controls for MSH4 protein in ready-to-use buffer for SDS-PAGE and Western blot analyses. These standards are used to identify the MSH4 protein in unknown samples on western blotting purposes. FabGennix International Inc. also carries limited quantities of antigenic blocking peptide for MSH4 antibodies. Please inquire about pricing and availability of these products. . FabGennix Inc. will also conjugate antibodies with fluorescent probes upon request at a reasonable cost. Please inquire for these custom services. A complete list of these and other items can be viewed at www.FabGennix.com.
For research use only, not for diagnostic or therapeutic use.
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