Radiation sensitive protein
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| RAD30-101AP | RAD30 antibodies | Rabbit | AP | Saccharomyces cerevisea | 100.0 ug | 150-500 ul |
$245.00
|
| FITC-RAD30 | FITC-conjugated antibodies | Rabbit | FITC-AP | Saccharomyces cerevisea | 100.0 ug | 150-500 ul |
$350.00
|
| P-RAD30 | Antigenic blocking peptide | n/a | Synthetic peptide | n/a | 250.0 ug | 100 ul |
$125.00
|
| PC-RAD30 | WB positive control for RAD30 | n/a | Protein in ready-to-use buffer | n/a | 0.0 ug | 150 ul |
$185.00
|
Saccharomyces cerevisiae Radiation sensitive protein 30 (Rad30) is the homolog of human DNA polymerase eta. The Saccharomyces cerevisiae RAD30 gene, a homologue of Escherichia coli dinB and umuC, is DNA damage inducible and functions in a novel error-free postreplication repair mechanism. Both human and yeast polymerase eta are responsible for error-free bypass of UV-induced cis-syn pyrimidine dimers and several other DNA lesions. The RAD30A gene encodes DNA polymerase eta and defects in the protein lead to the xeroderma pigmentosum variant (XP-V) phenotype in humans. The hypermutations in all 4 bases in immunoglobulin genes are the result of low fidelity DNA polymerases like Poleta encoded by Rad30 in S. cerevisea, RAD 30 is post-transalationally modified by Ubiquitinaion and proteosome mediated degradation. The biological half life for Rad30 is 20 minutes and is increased due ot proteosome defects (1). Other mutation inactivating proteins like Skp and F-box protein Ufo1 stabilize Rad30. UV radiation causes transient stabilization of Rad30 which tend to accumulate this protein in the cells. DNA polymerase eta, involved in the predominantly error-free bypass replication of DNA lesions, catalyzes the efficient and accurate synthesis of DNA opposite cyclobutane pyrimidine dimers; homolog of human POLH and bacterial DinB proteins.
The Rad30 gene resides on S cerevisea chromosome 4. There are wo variants of Rad30 proteins, Rad30-101AP antibodies were generated agisnt 632 amino acid protein from sacchromyces cerevisea. Rad30 contain several functional domain, including UmuC (26-309); Pol30 binding domain (625-632) and required Mg for its activity. Mutations at Aspartic acid 30 and or at 155, to Alanine abolished the DNA polymerse activity and several other mutations alter the enzymatic acitivity in different fashion.
The Anti-RAD30 selective antibodies were generated against conserved sequences from amino acid 284-300 of the Rad30 protein. The Rad30-selective antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded high affinity epitope-specific antibodies. The Rad30 antibodies label a protein of approximately 76-78kDa protein in Western blot using Western blot positive control for Rad30 (PC-Rad30). Anti-Rad30-selective antibodies are also available in affinity-purified form for confocal, Western blotting and immunocytochemical analyses. FabGennix Inc. will also conjugate antibodies with fluorescent probes upon request at extra charge. Limited quantities of antigenic peptide is also available (inquire before ordering). FabGennix Inc. also provides antibodies to other antibodies for epigenetics research, for a complete listing please visit www.FabGennix.com. FabGennix Int. Inc., will also provide Western blot positive controls for most of these antibodies in ready-to-use buffer for easy identification of respective proteins. Limited quantities of antigens are also available for blocking studies. Please inquire for their availability before ordering.
For research use only, not for diagnostic or therapeutic use.
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