Cyclic nucleotide gated channel
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| CNGb1-201AP | CNG1b antibodies | Rabbit | AP | r, h | 100.0 ug | 150-500 ul |
$245.00
|
| FITC-CNGb1 | FITC-conjugated antibodies | Rabbit | FITC-AP | r, h | 100.0 ug | 150-500 ul |
$350.00
|
| P-CNGb1 | Antigenic blocking peptide | n/a | Synthetic peptide | n/a | 250.0 ug | 100 ul |
$125.00
|
| PC-CNGb1 | WB positive control for CNG1b | n/a | Protein in ready-to-use buffer | n/a | 0.0 ug | 150 ul |
$185.00
|
Cyclic nucleotide-gated (CNG) channels play central roles in visual and olfactory signal transduction, these channels are activated by cAMP and are composed of at least 5 distinct members (CNG1-5). Native CNG channels are heteromeric complexes consisting of alpha subunit (CNG1-3) which can form functional channels by themselves and the modulatory beta subunits (CNG4-5). There at least two subtypes of CNG channel characterized (alfa and beta subunits) each expressed in multiple spliced variant form. Expression studies indicate that only CNG1-3 are able to form functional homooligomeric channels. The cone photoreceptor cells express only CNG alfa 2 subunit whereas rat olfactory sensory neurons has been shown to express aalfa3, alfa4 and beta 1 subunit. The CNGb1 is an alternate splice form of rod photoreceptor CNG beta 1a subunit (1). Single channel analyses on olfactory sensory neurons cilia suggest that CNG channel is assembled and composed of all three subunits in different combinations of a specific alfa subunit with various forms of an alternatively spliced beta subunit. Rat pineal gland CNG channels express three types of subunits, an alfa-subunit, two beta subunit splice variants (rCNG4.1 and rCNG4.2) and a hydrophilic glutamic acid-rich protein GARP in addition the CNG4 expression was also noted in arcuate periventricular and supraoptic nuclei of the hypothalamus (2).
CNG channels are expressed in several tissues and cell types suggesting a general function of these channels in a wide variety of cellular systems regulated by second messenger mediated pathways regulated by Ca+2 influx. The CNG channels in ROD photoreceptors cosist of CNG1 and CNG4 and olfactory neurons have CNG2, CNG4 and CNG5. In the cone photoreceptors only alfa subunit CNG3 is identified. Recently a new CNG6 subunit is identified in mouse retina which has highest similarity to CNG4 beta subunit (3). The CNG channels are also involved in spermatogenesis and steroidogenesis in testis. The CNGs are expressed along with PKGI, soluble guanylate cyclases (sGC) in spermatids. The CNG beta1 subunit is a 1339 aa protein containing several functional motifs and posses a single transmembrane domain. The CNGb1 is located on chromosome 19p13.
The Anti-CNGb1-selective antibodies were generated against conserved sequences that are unique to human and rat CNGb1protein. The CNGb1-selective antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded epitope-specific antibodies. The CNGb1 antibodies label a 160kDa protein in Western blot using Western blot positive control for rat CNGb1 (PC-CNGb1). Anti-CNGb1-selective antibodies can be conjugated to FITC or other fluorophores for direct application in IHC and confocal microscopy at a nominal charge. Limited quantities of Western blot positive controls for CNGb1 (PC-CNGb1) and antigenic blocking peptide for CNGb1 antibody are also available, please inquire availability before placing orders. FabGennix Inc. also provides antibodies to many transporter and ion channel proteins, for a complete listing please visit www.fabgennix.com. FabGennix Inc employs cyclic peptide methodology for generating antibodies, which results in higher titer and specificity.
For research use only, not for diagnostic or therapeutic use.
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