Transducer of regulated cAMP response element-binding protein 2 (TORC-2) is a member of TORC family, the coactivators of the cAMP-responsive factor CREB; it is located at chromosome 1q21.3 (1). TORC-2 is a key regulator of fasting glucose metabolism. In mice, TORC-2 overexpression strongly induces glucose output in primary hepatocytes whereas knockdown of TORC-2 significantly reduces glucose output and gluconeogenic gene expression (2). TORC-2, along with other transcriptional regulators, regulates insulin receptor substrate 2 (IRS2) expression therefore limiting glucose output from the liver by inducing IRS2 (3). It may also contribute to the salutary effects of calorie restriction on glucose homeostasis in liver and other tissues (4).

TORC-2 regulates the expression of specific genes such as the steroidogenic gene, StAR and, it is also a coactivator for TAX activation of the human T-cell leukemia virus type 1 long terminal repeats (5). Inhibition of TORC-2 inhibits cell cycle progression, cell proliferation, and anchorage dependent growth in MCF7 breast cancer and PC3 prostrate cancer cells (6). Therefore, TORC-2 inhibitors might be useful for treating cancers. TORC-2 is highly expressed in thymus, HEK293T cells, insulinomas, spleen, ovary, muscle, lung and, B and T lymphocytes. TORC-2 is approximately an 85 kDa protein (693 amino acids).

The PTORC2-selective antibodies were generated against purified Phosphorylated Transducer of CREB protein 2 (PTORC2). PTORC2-antibodies are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. FabGennix Int. Inc., will also provide limited quantities of antigenic blocking protein for TORC2-101AP antibody. Antibodies to TORC2 (TORC2-101AP) will label ~85kDa protein  in Western blot positive control samples for TORC2 and several other tissues.  FabGennix Inc. will also conjugate antibodies with secondary enzymes (alk-Pase or HRP) or fluorescent probes upon request at nominal cost.

For research use only, not for diagnostic or therapeutic use.