Glucose Transporter 4
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| Glut-401AP | Affinity purified Glut 4 antibody | rabbit | AP | R, M | 100.0 ug | inquire |
$245.00
|
| Glut4-FITC | FITC-conjugated Glut 4 antibody | rabbit | AP | R, M | 100.0 ug | inquire |
$350.00
|
| P-Glut 4 | Blocking peptide for Glut-401AP | rabbit | AP | R, M | 100.0 ug | inquire |
$135.00
|
| PC-Glut 4 | WB positive control | rabbit | AP | R, M | 100.0 ug | inquire |
$145.00
|
Seven types of glucose transport carrier proteins designated as Glut 1-7 facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encodes at least 7 proteins exhibiting high degree of amino acid homology (45%-65%) all in the molecular weight range of 40-60 kDa. Individual members of the Glut family have predicted secondary structure characteristic of 12 membrane spanning domains of other transport carriers. Majority of the differences in sequence homology in Glut proteins occur at 4 hydrophilic domains that may play a role in distinct tissue-specific pattern of expression and targeting. All Glut proteins are glycosylated at or near the C-terminus and are present on either cell surface or in intracellular sites. Some transporters exhibit dynamic trafficking between intracellular storage sites and plasma membranes in response to various stimuli. In some tissues Glut proteins are asymmetrically distributed between apical and basolateral membranes as in blood brain barrier and blood testis barriers.
The Glut family-selective antibodies were generated against unique C-terminal peptides characteristics of a particular Glut family. FabGennix Inc. has generated highly specific rabbit anti-Glut 5 polyclonal antibodies utilizing mostly C-terminal sequences. These antibodies have been fully characterized for cross reactivity with in the Glut family and with other cellular proteins. FabGennix Inc. has produced antibodies to multiple epitopes on the same protein that will facilitate studies utilizing interspecies cross reactivity.
FabGennix Inc. provides Glut 4 Western blot positive control in “ready-to-use” SDS-PAGE sample buffer. The Glut 4 positive control appears as a diffuse band of 47-52 kDa and co-migrates with rat adipocyte membrane Glut 4 protein on a 10% SDS-PAGE.
Concentration: IgG concentration range from 0.75 ug/ul-0.95ug/ul for Glut-401AP.
Applications:
ELISA Antibody dilution 1:4,000 for ELISA or DOT blot with solublized hippocampal plasma membranes.
W.B Antibody dilution 1:1,000 for WB using 10 mg of solubilized abluminal membranes from rat jejunum as antigen.
IMM 2 ml of Glut-401AP or 2.5 ml of Glut-400P will immunoprecipitate 80-85% Glut 4 from 250 mg rat solubilized adipocyte membranes. IHC; n.d
Reactivity The antibodies (Glut-400P and Glut-401AP) label glucose transporter (Glut 4) species in rat skeletal muscle and adipocyte membrane as an average 47-52 kDa molecular weight protein.
Protocols: Standard protocol for various applications (Western blot; immunoprecipitation and immunohistochemistry) of this antibody is provided with the product specification sheet, however, FabGennix Inc. strongly recommends investigators to optimize conditions for use of this antibody in their laboratories.
WB, Western Blot analyses; IMM, Immunoprecipitation; IHC, Immunohistochemistry, n.d, not determine.
Form/Storage: The antiserum is supplied in antibody stabilization buffer with 0.02% azide. The purified antibodies are isolated from immobilized antigen affinity column. The affinity pure antibodies are supplied as 0.5-1 mg/ml IgG in antibody stabilization buffer. For long-term storage of keep at -20oC. FabGennix Inc. does not recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi use antibody dilution buffer (Cat # DiluOBuffer). Working solutions of antibodies in DiluOBuffer should be filtered through 0.45m filter after every use for long-term storage.
References:
1. Meuckler M. M. NIPS, 10, 22-29, 1995.
2. Farooqui S. M., Bagdadi A. F., Rhett S., and O’Donnell J. M.,. Biochem. Biophys. Res. Comm. 236, 407-412, 1997.
Note: Do not heat or boil Glut 1-PC samples. This product is “ready to use” for electrophoresis. After thawing at room temperature. Repeated freezing and thawing may result in appearance of higher molecular weight immunoreactive bands.
* For users who may require large amounts of Glut-400P/401AP, please enquire about bulk material discounts.
This Product is for Research Use Only and is NOT intended for use in humans or clinical diagnosis. 050801-0020SF1001Z-rev10.01
For research use only, not for diagnostic or therapeutic use.
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