Chemokine C-C Receptor 7

Chemokine (Chemoattractant Cytokines) are small peptides that are potent activators and chemo-attractants for leukocyte subpopulations and other non-hemopoietic cells.  Chemokine receptors (CCR) belong to the super-family of G protein-coupled receptors (GPCR), which regulate the trafficking and activation of leukocytes, and operate as co-receptors in the entry of HIV-1 and proliferation and migration of immature neurons, glia and their precursors (1).  Furthermore, chemokine receptors participate in the etiology and progression of various brain disorders, including AIDS dementia, neuro-inflammatory disease and neuroplasia, making them important potential therapeutic targets in these cases several subtypes of CCR have been characterized (Table1).

	Type	Cat #	Expression
1	CCR1	CCR-101AP	Melanoma, leukocytes, microglia, EC, podocytes
2	CCR2	CCR-201AP	Intestinal microvascular cells
3	CCR3	CCR-301AP	Microglia, podocytes
4	CCR4	CCR-401AP
5	CCR5	CCR-501AP	Podocytes,
6	CCR6	CCR-601AP
6	CCR7	CCR-701AP

 Activation of naïve T cells by specific antigen and cytokines, up-regulate cell adhesion molecules (CAM) as well as chemokine receptors on their surface, which directs them to migrate towards the inflamed tissues.  Chemokine receptor type 3 (CCR3) is induced during T cell activation and CCR3 positive cell are enriched at the inflammatory site (2).  On the contrary, glucocorticoid dexamethasone has potent anti-inflammatory effects, yet it up-regulates expression of CCR4 but not that of CCR1 and CCR3 (4).  The chemokine receptor CCR7 and CXCR4 have been suggested to play important role in cancer invasion and metastasis.  CCR7 expression may be associated with lymph node metastasis; moreover, the expression of these receptors can serve as an indicator of poor prognosis in patients with cervical and breast cancer (5).  CCR7 and its ligands CCL19 and CCL21 are a chemokine system related to mononuclear cell migration and antigen presentation, and are suggested to play a key role in several autoimmune disordersThe chemokine receptor 7 protein is approximately 52-54kDa protein which has 7 transmembrane domains, characteristic of G-protein coupled receptors.  The protein has putative N-glycosylation sites near the extra cellular N-terminal end of the proteins.  The CCR7 protein also has a large 3^rd intra cellular loop which interacts with G-proteins and other signal transduction molecules.  The short carboxy terminal is intracellular and has putative post-translational sites.  The Anti-CCR7-selective antibodies were generated against amino terminal end of the protein that is conserved but unique for CCR7 receptor.  This epitope is not expressed in any other CCR or CXCR receptors family members.  The mono-epitope specific polyclonal antibodies are affinity purified on an immobilized antigen based affinity chromatography.  The epitope-specific antibodies strongly label CCR7 chemokine receptor in CCR7 western blot positive  control samples.  FabGennix provides subtype selective affinity purified antibodies to various other CCR and CXCR family members, for a complete listing plese visit www. FabGennix.com. Anti-CCR-selective antibodies can also be made available in the form of fluorescent conjugates for direct application in IHC upon request at extra charge. FabGennix, Inc., will also provide Western blot positive controls and limited quantity of antigenic blocking peptides for most of these antibodies in ready-to-use buffer for easy identification of respective proteins are also available, please inquire about their availability before ordering.

 

For research use only, not for diagnostic or therapeutic use.