Guanylate Cyclase B
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| PGCB-201AP | Particulate Guanylate Cyclase B | Rabbit | Polyclonal | r, m, h | 100.0 ug | 175-500ul |
$245.00
|
| FITC-PGCB | FITC-conjugated PGCB antibody | Rabbit | FITC-conjugated antibody | r, m, h | 100.0 ug | 175-500ul |
$350.00
|
| P-PGCB | Antigenic blocking peptide | n/a | Synthetic peptide | n/a | 250.0 ug | 100ul |
$125.00
|
| PC-PGCB | WB positive control for PGCB | n/a | Protein in ready-ro-use buffer | n/a | 0.0 ug | 150ul |
$185.00
|
| Sampler-PGCB | Sampler pack of any 6 PGC antibodies | Rabbit | Polyclonal | r, m, h | 50.0 ug | 175-500ul |
$695.00
|
Cyclic GMP (cGMP), a key messenger in several signal transduction pathways, the intracellular levels of cGMP are maintained by the activity of opposing enzymes: synthesizing gualylyl cyclases (GC) and hydrolyzing phosphodiesterases (PDEs). The synthesizing enzymes (GCs) are found in two forms: cytosolic (soluble) and membrane-bound (particulate), while they share similar structural characteristics, they differ in their mechanisms of physiological regulations. Most importantly, soluble GC (sGC) contains a heme group and binds NO that activates the enzyme, while particulate GC (PGCs) are stimulated by natriuretic peptides. Particulate forms of guanylyl cyclases have been shown to function as natriuretic peptide receptors. In response to G-protein coupled receptor stimulation, the cGMP can be produced from GTP by either soluble guanylate cyclase (sGC), or by PGC. The sGC are heterodimers (a & b polypeptide chains), that are stimulated by nitric oxide and carbon monoxide or by particulate membrane-bound guanylyl cyclases which are activated by a complex mechanism by natriuretic peptides. PGCs have 7 different isoforms, PGC-A through PGC-G and are expressed in most tissues in isoform specific manner (See Table 1). There is significant structural homology among various PGCs, there is a large N-terminal extracellular domain (ECD), a single TMD and a large intracellular domain with protein kinase activity (KLD), a C-terminal catalytic domain (CD) and in between is a dimmerization domain (DD). Guanylyl cyclase B (PGC-B) is a receptor for B type brain natriuretic peptide (BNP) and is 78% identical to PGCA in the ECD but only 43% in the CD. The binding of a ligand to the extra-cellular domain of PGC-B triggers signal that control central and peripheral cardiovascular homoeostasis. Both PGC-A and PGC-B are phosphorylated at Serine residues in the KLD (2). Non-ionic detergents stimulated particulate guanylate cyclase activity in cerebral cortex of rat 8- to 12-fold while stimulation of soluble enzyme was 1.3- to 2.5-fold (2).
The Anti-PGC-B-selective antibodies were generated against conserved sequences near the C-terminal end of the protein that are unique to PGC-B protein. The PGC-B-selective antibodies are affinity purified against immobilized antigen based affinity chromatography that yielded epitope-specific antibodies. The PGC-B antibodies label a 120-122 kDa protein in various tissues including brain, kidney, testis and spleen. Anti-PGC-B-selective antibodies are also available in affinity-purified form for confocal, WB, IHC analyses. FabGennix Int. Inc. will also conjugate antibodies with fluorescent probes upon request at extra charge.
FabGennix Inc. also provides antibodies to other family members of the particulate GC (PGA, C, D, E, F and G) and to various adenylate cyclases (AC-1 through 9). FabGennix Inc employs cyclic peptide methodology for generating antibodies, which results in higher titer and specificity (6). FabGennix, Inc., will also provide Western blot positive controls for most of these antibodies in ready-to-use buffer for easy identification of respective proteins. Limited quantities of antigens are also available for blocking studies. Please enquire for their availability before ordering.
For research use only, not for diagnostic or therapeutic use.
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