p21-activated protein kinase
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| PAK-101AP | PAK antibodies | Rabbit | AP | r, m, h, c, monk | 100.0 ug | 150-500 ul |
$245.00
|
| FITC-PAK | FITC-conjugated antibodies | Rabbit | FITC-AP | r, m, h, c, monk | 100.0 ug | 150-500 ul |
$350.00
|
| P-PAK | Antigenic blocking peptide | n/a | Synthetic peptide | n/a | 250.0 ug | 100 ul |
$125.00
|
| PC-PAK | WB positive control for PAK | n/a | Protein in ready-to-use buffer | n/a | 0.0 ug | 150 ul |
$185.00
|
A brain serine threonine protein kinase is a target for p21 ras related proteins Cdc42 and Rac1. p21-activated kinases (PAKs) bind to and are activated by Rho family GTPases such as Cdc42 and Rac. These GTPases play key roles in regulating cell polarity, stress responses, and cell cycle progression (1). Stiochiometeric analyses using purified Rac and PAK suggest that Rac binds to PAK with high affinity in a 1:1 ratio. The kinase complexes specifically with aGTP bound activated p21, inhibiting the p21 activity and leading to activation and auto-phosphorylation of p65pak (2). Three isoforms of p21-activated serine/threonine kinases, PAK68, PAK65 and PAK62, were found in both cytosolic & membrane fraction, & their contents were significantly increased during induced retinoic acid differentiation of human promyelocytic leukemia HL60 cell (3). The content of PAK65 or Rac is not evident in plasma membrane fraction of cells form un-differentiated cells (2).
Constitutively activated Pak1 also suppress the activity of scavenger receptor class B, type 1 (SB-B1) in macrophage cell line RAW264.7, an action similar to LPS (4). Mutational studies with PAK1 also suggest that SR-B1 receptor down regulation involves binding to Rac and Cdc42. In mammalian cells, hyper osmotic shocks can induce apoptotic cell death. It is suggested that hyper osmotic shock can induce cleavage for PAK2 to produce a 36kDa kinase, which is the result of DNA fragmentation and activation of ICE/CED-3 family cysteine proteases termed caspases-3.
The p21activated PAK-selective antibodies were generated against synthetic peptide corresponding to residues 484-502 corresponding to human lymphocyte p65PAK. The antibodies to p65PAK (PAK-101AP) are affinity purified over immobilized antigen based chromatography. The purified antibodies are stabilized in antibody stabilization buffer containing preservatives. FabGennix Int. Inc., will also provide Western blot positive controls for human p65PAK in ready-to-use buffer. Please enquire about pricing and availability of the Western blot positive controls. Other kinase related antibodies that are carried by FabGennix Int. Inc. include p21 activated protein kinase (p65pak), Anti-calmodulin dependent kinase gamma, Anti-MER tyrosine kinase (MERTK) and Anti-auto activated protein kinase. FabGennix Inc. will also conjugate antibodies with fluorescent probes, magnetic beads or to various affinity matrices upon request at a reasonable cost.
For research use only, not for diagnostic or therapeutic use.
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