E2F2 Transcription Factor

E2F transcription factors are important key regulators of cell growth control. There are 5 types of E2F transcription factors (E2F1-E2F5) that are known to important role in a number of pathological conditions. A double mutant of E2F1/E2F2 mouse has shown to develop non-autoimmune insulin-deficient diabetes and exocrine pancreatic dysfunction with exocrine dysplasia (1). The E2F family of transcription factors consists of two subgroups termed E2F and DP transcription factors that binds DNA cooperatively with dp proteins through the E2 recognition site, TTTCC/GCGC, found in the promoter region of a number of genes. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Deregulation of the Rb-E2F1 pathway also results in the activation of p53 and the promotion of apoptosis, and this led to the suppression of tumor development (2).
 E2F proteins are also involved in the regulation of human intestinal epithelial cell proliferation and development. The expression of E2F1 is decreased and E2F4 protein is sequestered in the cytoplasm during G(0) arrest associated with serum deprivation, confluence, and terminal differentiation of intestinal cells (3). E2F1 deficiency also impairs murine spermatogenesis and augments testicular degeneration. E2F1 is a 437 amino acid (53kDa) protein. The E2F2-selective antibodies were generated against three antigenic sequences form N-, midregion and C-terminal end of the protein. The antibodies E2F2 are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. FabGennix Int. Inc., will also provide limited quantities of antigenic blocking peptides for E2F2 antibodies. FabGennix International Inc. also carries antibodies that are related to E2F pathways include p14ARF, p27kip, Her2, Her3, ACK1, p21ras, NP2, and more. FabGennix Inc. will also conjugate antibodies with fluorescent probes upon request at a reasonable cost.