Napsin A
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| NAP-101AP | Napsin A antibodies (N-terminal) | Rabbit | AP | r, m, h | 100.0 ug | 200 ul |
$245.00
|
| NAP-112AP | Napsin A antibodies (C-terminal) | Rabbit | AP | r, m, h | 100.0 ug | 200 ul |
$245.00
|
| FITC-NAP100 | FITC-conjugated N-terminal antibodies | Rabbit | FITC-AP | r, m, h | 100.0 ug | 200 ul |
$350.00
|
| FITC-NAP110 | FITC-conjugated C-terminal antiboides | Rabbit | FITC-AP | r, m, h | 100.0 ug | 200 ul |
$350.00
|
| P-NAP100 | N-terminal antigenic blocking peptide | n/a | Synthetic peptide | n/a | 250.0 ug | 100 ul |
$125.00
|
| P-NAP110 | C-terminal antigenic blocking pepitde | n/a | Synthetic peptide | n/a | 250.0 ug | 100 ul |
$125.00
|
| PC-NAP | WB positive control for Napsin A | n/a | Protein in ready-to-use buffer | n/a | 0.0 ug | 150 ul |
$185.00
|
Napsin A gene codes for two aspartic proteinases precursor generally expressed in lungs and kidneys and its translational product is fully functional, glycosylated aspartic proteinase containing a RGF motif and an additional 18 residues at its C-terminal end. The Napsin B gene is expressed in exclusively in cells of the immune system and is expressed in several polymorphic form (1). Members of the aspartic protease family have been implicated in cancer progression. The aspartic protease napsin A is expressed in type II cells of the lung, where it is involved in the processing of surfactant protein B (SP-B). Napsin A expression in kidney is decrease in adenocarcinomas this result is consistent with earlier observations of loss of napsin A in high grade lung carcinomas (2). Mutation in the aspartic acid residue resulted in complete loss of enzymatic activity but it did not influence glycosylation, processing and intracellur localization.
Re-expression of napsin A in HEK-293 cells inhibit the tumor growth by mechanism independent of aspartic proteinase activity (2).
Interstitial lung disease (ILD) ail lung in children represent a rare heterogeneous group of respiratory disorders characterized by restrictive lung disease and diffuse infiltrates. Mutations in the surfactant proteins (SP-A, SP-B, SP-C and SP-D) and in the ABC binding cassette protein A3 (ABCA3) are found to be associated with pediatric ILD (3). While SP-A and SP-D play essential roles in the pulmonary immune defense, SP-B and SP-C lower the surface-tension and facilitate formation and stabilization of the surfactant film in young lungs. SP-B is expressed in type II non-ciliated bronchiolar epithelial cells in pre-pro-protein of 381 amino acids. After removal of 23amio acids form N-terminal end the pro-protein is proteolytically processed at the amino and carboxy terminal ends to mature SP-B protein of 79 amino acids by a cystein protease, cathapsin H and aspartylprotease Napsin (4). Napsin A is highly expressed in type II non-ciliated epithelial cells and is implicated in processing of the SP-B and SP-C pro-peptides (4).
The Napsin A-selective antibodies were made in rabbits using synthetic peptides corresponding to human Napsin A protein. Anti-Napsin A antibodies label a 50-53kDa protein in PC-NAP samples. Anti-Napsin A-selective antibody is available in affinity-purified form isolated by immobilized affinity chromatography for confocal, Western blotting and immunocyto emical analyses. A FITC-conjugated Napsin antibody is also available for direct application in IHc and confocal applications. FabGennix Inc. will also conjugate antibodies with other fluorescent probes upon request at a nominal charge. Limited quantitative of antigenic blocking peptides for napsin A antibody is also available. FabGennix Inc., has a wide selection of antibodies to protein klinases, phosphatases, proteinases and convertases, for a complete listing please visit www.FabGennix.com. FabGennix, Inc., will also provide Western blot positive controls for most of these antibodies in ready-to-use buffer for easy identification of respective proteins. Limited quantities of antigens are also available. Please enquire for their availability before ordering.
For research use only, not for diagnostic or therapeutic use.
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| FabGennix Product Used | Authors, Year, Journal, Title | Technique used | Comments |
|---|---|---|---|
| Synaptophysin | Morita I. et. al., 2009 J. Biological Chemistry 284: 30209-30217, 2009 HNK-1 Glyco-epitope Regulates the Stability of the Glutamate Receptor Subunit GluR2 on the Neuronal | WB, IHC | |
| Synaptophysin, PDE4D | Li Y., et. al., 2009 Proc. Natl. Acad. Sciences, 106: 21918-21923, 2009 Regulation of amygdalar PKA by â-arrestin-2/phosphodiesterase-4 complex is critical for fear conditi | WB, IHC | |