Sestrin 3
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| SESN-301AP | Sestrin 3 antibodies | Rabbit | AP | r, m, h | 100.0 ug | 150-500 ul |
$245.00
|
| FITC-SESN3 | FITC-conjugated antibodies | Rabbit | FITC-AP | r, m, h | 100.0 ug | 150-500 ul |
$350.00
|
| P-SESN3 | Antigenic blocking peptide | n/a | Synthetic peptide | n/a | 250.0 ug | 100 ul |
$125.00
|
| PC-SESN3 | WB positive control for SESN3 | n/a | Protein in ready-to-use buffer | n/a | 0.0 ug | 150 ul |
$185.00
|
Sestrin 3(SESN3), located at human chromosome 11q21, belongs to the sestrin family of proteins. These proteins are cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense (1). This family of proteins is only present in multicellular organisms ranging from nematodes to mammals. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes (2). Peroxiredoxins are the enzymes that metabolize peroxides. Expression of these proteins is regulated by p53, a tumor suppressor protein (3). Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity (4). Recently it was reported that sestrin 3 may play an important role in AKT induced increase in ROS and it might be a promising target in selectively killing cancer cells containing high levels of AKT activity (5).
AKT is a protein kinase that is hyperactivated in cancer. This hyperactivity leads to an increase in intracellular ROS mainly by inhibiting the expression of ROS scavengers downstream of FoxO, such as sestrin 3 (4). This phenomenon was supported by the fact that AKT deficient cells contained high levels of sestrin 3 leading to low levels of ROS. Furthermore, downregulation of sestrin 3 restored ROS levels in these cells (5). Hyperactivation of AKT has been shown to promote uncontrolled cell growth and also protect cells from apoptosis (6). Inhibition of mRNAs from sestrin 3 as well as from sestrin 1 has also been shown to substantially increase intracellular ROS levels. This increase in ROS is linked to Ras-induced mutagenesis which is one of the most common events in cancer (7). Sestrin 3 is approximately a 38.5kDa protein (480 amino acids) in human and 59kDa protein (492 amino acids) in rat and mouse.
The SESN3-selective antibodies were generated using peptide corresponding to human Sestrin 3 protein. SESN3-antibodies are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. FabGennix Int. Inc., will also provide limited quantities of antigenic blocking protein for SESN-301AP antibody. Antibodies to SESN3 (SESN-301AP) will label ~59kDa protein in Western blot positive control samples for SESN3 (PC-SESN3) and several other tissues. FabGennix Inc. will conjugate this and other antibodies from its catalog to either secondary enzymes (alk-Pase or HRP) or fluorescent probes at a nominal cost upon request. FabGennix also provides custom antibody production services for researchers that are looking for high affinity mono and polyclonal antibodies in various species. We specialize in making application specific antibodies that are useful in IHC, confocal and other applications where native antigen is detected. For a complete listing of all FabGennix antibodies please visit www.Fabgennix.com.
For research use only, not for diagnostic or therapeutic use.
Data Sheets
MSDS
General Information
Citations
Search FabGennix Product Citation Data Base
Search