Thirteen types of glucose/fructose transport carrier proteins designated as Glut 1-13 facilitate glucose/fructose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encodes at least 13 proteins exhibiting high degree of amino acid homology (45%-65%) all in the molecular weight range of 40-60 kDa. Individual members of the Glut family have predicted secondary structure characteristic of 12 membrane spanning domains of other transport carriers. Majority of the differences in sequence homology in Glut proteins occur at 4 hydrophilic domains that may play a role in distinct tissue-specific pattern of expression and targeting. All Glut proteins are glycosylated at or near the C-terminus and are present on either cell surface or in intracellular sites. Some transporters exhibit dynamic trafficking between intracellular storage sites and plasma membranes in response to various stimuli. In some tissues Glut proteins are asymmetrically distributed between apical and basolateral membranes as in blood brain barrier and blood testes barriers.
The Glut family-selective antibodies were generated against unique C-terminal peptides characteristics of a particular Glut family. FabGennix Inc. has generated highly specific rabbit anti-Glucose transporter polyclonal antibodies utilizing mostly C-terminal sequences. These antibodies have been fully characterized for cross reactivity within the Glut family and with other cellular proteins. FabGennix also has western blot positive controls for various glucose transporters in ready-to-use buffer and antigenic blocking peptides for immune blocking experiments. FabGennix Inc., will conjugates these antibodies to fluorophores and secondary enzymes for a nominal charge. Please contact customer service for more details.
FabGennix Inc. provides Glut 9 Western blot positive control in “ready to use” SDS-PAGE sample buffer. The Glut 9 positive control appears as a diffuse band of 47-52 kDa and co-migrates with rat kidney Glut 9 protein on a 10% SDS-PAGE.
For research use only, not for diagnostic or therapeutic use.
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