Sterol regulatory element binding transcription factor 1
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| SREBF-101AP | Affinity Purified SREBF1 antibodies | Rabbit | AP | r, m, h, others | 100.0 ug | 150-500 ul |
$245.00
|
| FITC-SREBF1 | FITC-conjugated SREBF1 antibodies | Rabbit | FITC-AP | r, m, h, others | 100.0 ug | 150-500 ul |
$350.00
|
| P-SREBF1 | Antigenic blocking peptide for SREBF1 | n/a | Synthetic peptide | n/a | 250.0 ug | 100 ul |
$125.00
|
| PC-SREBF1 | WB positive control for SREBF1 | n/a | Prtoein in ready-to-use buffer | n/a | 0.0 ug | 150 ul |
$185.00
|
Sterol regulatory element binding-protein 1 (SREBP1/SREBF1) belongs to the SREBP family of transcription factors which are members of the basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factor family (1). SREBP family of transcription factors play a vital role in regulating genes involved in biosynthesis and uptake. More specifically, they modulate the expression of enzymes implicated in the synthesis of cholesterol, fatty acids, triacylglycerol and phospholipids (2). These proteins are synthesized in their inactive form in the ER where they form complex with SREBP cleavage activating protein (SCAP). SCAP restricts SREBPs to the ER under high cholesterol concentrations. It is only under low concentrations that SREBP is transported to the golgi apparatus for activation (1). Here, SREBPs undergo two proteolytic cleavage steps to release the active transcription factor known as nuclear SREBP (nSREBP). Once activated, SREBPs can translocate to the nucleus and bind to sterol response elements (SRE) in the promoter/enhancer regions of target genes (1).
Similarly, the activated SBREBF1 binds to the sterol regulatory element-1 (SRE1) (3). This protein plays a key role in regulating genes for hepatic lipid synthesis (4). It has been reported that cells that are under stressful conditions, such as serum starvation, have an elevated level of SREBF1. Therefore, this protein is also implicated in insulin resistance, diabetes and other diet-related diseases (5). Elevated levels of SREBF1 were also seen in senescent cells and in liver tissues of aging rats (6). Recently it was reported that cells over-expressing SREBF1 are involved in the regulation of myogenesis. SREBF1 is located on human chromosome 17p11.2. It is expressed in a wide variety of tissues, but abundantly expressed in only liver, adrenal gland, and fetal lung tissues. SREBF1 is approximately a 137.6kDa protein (1147 amino acids).
The SREBF1 antibodies were generated using peptide corresponding to human SREBF1 protein. SREBF1 antibodies are affinity purified over immobilized antigen based affinity chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. FabGennix Int. Inc. will provide limited quantities of antigenic blocking protein for competition assays involving SREBF1 antibodies. Antibodies to SREBF1 (SREBF-101AP) will label ~137.6kDa protein in Western blot positive control for SREBF1 (PC-SREBF1) and several other tissues. FabGennix Inc. will conjugate this and other antibodies from its catalog to either secondary enzymes (alk-Pase or HRP) or fluorescent probes at a nominal cost upon request. FabGennix also provides custom antibody production services for researchers that are looking for high affinity mono and polyclonal antibodies in various species. We specialize in making application specific antibodies that are useful in IHC, confocal and other applications where native antigen is detected.
For research use only, not for diagnostic or therapeutic use.
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