Phosphodiesterase Type 2 antibodies

yclic nucleotides are important intracellular second messengers which play important role variety of signal transduction process.  The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases.  One of the many phosphodiesterases that compartmentalized and hydrolyze cAMP and cGMP in to AMP and GMP respectively are phosphodiesterase type 2  There are two member of PDE2A gene are cloned and designated as PD2A and PDE2B (Rascon et. al., 2002).  Each of the PDE2A and PDE2B genes are 2793 Bases and 930 amino acids with an apparent MW of 100 kDA.  The catalytic domain of PDE2A is homologous to all other 11 PDE family members.  The PDE2A has specificity for cAMP with Km of 2.4 uM and its activity is modulated by the presence of cGMP.  In contrast, the PDE2B does not hydrolyze cGMP nor its activity is altered by the presence of cGMP.   Western blot analyses of PDE2A reveals a variety of tissues including neocortex, cerebellum, heart, kidney lungs, pulmonary artery and skeletal muscle (Sadhu et. al., 2002).  The PDE2A expression was evident in venous arterial endothelial cells but not in arterial endothelial cells (Sadhu et. al., 1999).  The PDE2A expression was also noted in corpus cavernosum along with a wide repertoire of other PDEs enzymes (Kuthe et. al., 1999).    

 FabGennix Inc. also provides antibodies to other PDE family members.  The PDE-selective antibodies are PDE-selective, family subtype-selective and family-subtype-variant selective antibodies for detailed analyses of cyclic nucleotide signaling pathways.  We have generated two PDE2A-selective antibodies (PD2A-101AP, FITC-PDE2A and Biotin-PDE2A) that were generated from epitopes close to C-terminal regions.  The polyclonal antibody (PD2A-101AP) labels a 103 kDa PDE2A protein in rat brain and other tissues.  The PDE2A-specific antiserum has no cross reactivity against PDE2B or with other PDE family members tested so far.  

The PDE2A-selective antibodies (PD2A-101AP ) was generated using cyclic peptide methodology that results in higher titer and specificity.  PDE2A-selective antibody is also available in affinity purified form for confocal, Western blotting and immunocytochemical analyses.  The affinity purified antibodies are also conjugated to FITC and Biotin for direct applications in IHC FACS assay.  FabGennix Inc. will also conjugate antibodies with enzymes, fluorescent probes upon request at extra charge.

For research use only, not for diagnostic or therapeutic use.