Ca-Calmodulin Kinase I gamma

Ca2+/calmodulin-dependent protein kinase I (CaM kinase I) was first purified from bovine brain based on its ability to phosphorylate the synaptic vesicle protein, synapsin I at site 1.  CaM kinase I is similar to other known protein kinases with respect to presence of all nine invariant amino acids conserved in the catalytic domain of this class of enzymes. Rat, human and mouse cDNA clones were then isolated and expressed in various host cell lines.  The rat Ca/Calmodulin-dependent kinase cDNA encodes a protein of 331 amino acids with a calculated Mr. Wt. of 37,545.  Human Ca(2+)-calmodulin (CaM) dependent protein kinase I (CaMK-I) encodes a 370 amino acid protein with a calculated Mr. Wt. of 41,337.  The mRNA for CaMK-1 (1.5 kb) is expressed in many different human tissues and is derived from a single gene located on human chromosome 3. CaMK-I activity is directly regulated (increases) by calcium and calmodulin.  Various truncation studies reveled that residues 295-306 are sufficient to maintain CaMKI in an auto-inhibited state (2).  CaMKI was phosphorylated on Thr177 and its activity enhanced approximately 25-fold by CaMKI kinase in a Ca(2+)-CaM dependent manner (3).  Three subtype variants have been isolated for CaMK-1, alpha, beta and gamma.  High concentrations of the kinase mRNA were found in all regions of the brain with frontal cortex showing the greatest level (2).  

Three Anti-CaMK-1-selective antibodies were generated using a GST fusion protein and 2 peptide sequences from the caMK-1 gene.  The CaMK-1-selective antibodies were affinity purified against immobilized antigen based affinity chromatography and are represented as pure IgG fractions stabilized in antibody stabilization buffer.  The polyclonal antibodies strongly label a 36-38 kDa protein in Calmodulin kinase 1 gamma Western blot positive control sample.   The CaMK-1 antibodies are also available as HRP conjugate for confocal, Western blotting and immunocytochemical analyses as special order item.  FabGennix Inc. will also conjugate antibodies with fluorescent probes upon request at extra charge.   FabGennix Inc. also provides antibodies against other protein kinases including MER Tyrosine kinase proteins (MERTK), CD3, Auto Activated protein kinases, Casein Kinase 2, Myelin Basic Protein  kinase 1, Myelin Basic Protein Kinase 2, p65^PAK and Cytosolic Protamine Kinase.  These antibodies are available from FabGennix Int, Inc., as affinity purified form in 100 ug packing.   FabGennix, Inc., will also provide Western blot positive controls for most of these antibodies in ready-to-use buffer for easy identification of respective proteins.  Limited quantities of antigens are also available.  Please enquire for their availability before ordering.

For research use only, not for diagnostic or therapeutic use.