Presenilin stabilizing protein

Accession: Q8WW43; Alternate Nomenclature:  Anterior Pharynx Defective 1homolog B; Gamma-secretase subunit APH-1B, Aph-1beta, Presenilin-stabilization factor-like.

Regulated intramembrane proteolysis by γ-secretase cleaves proteins in their transmembrane domain and is involved in important signaling pathways.  gamma-Secretase catalyzes the intramembrane proteolysis of Notch, beta-amyloid precursor protein, and other substrates as part of a new signaling paradigm and as a key step in the pathogenesis of Alzheimer’s disease.  The intra-membrane proteolysis of amyloid-beta precursor protein (APP) and Notch along with many other cellular proteins is mediated by gamma-secreatase complex which is primarily composed of presenilin and nicastrin. The gamma secretase complex is formed by direct physical interaction of 4 proteins to form heterodimers including presenlin-2 (PEN-2), highly glycosylated nicastin, and mammalian Aph-1 that are genetically linked to gamma secretase. Coexpression of all 4 protein leads to a marked increase in presenilin heterodimers, fully glycosylation of nicastrin and enhanced gamma secretase activity (1).  This Aph1 plays an important role in initial assembly and maturation of presenilin, nicastrin complexes. The gamma secretase complex plays a role in Alzheimer’s disease and cancer progression. Specific inactivation of Aph1b in mouse Alzhiemer’s model showed improvement in Alzhiemer’s disease relevant phenotypic features without any Notch related side defects.

Mammalian and C. elegans Aph1b is a very conserved protein, comprised of 257 amino acid with 7 TMD located on chromosome 15p22.2. The Aph1b protein is expressed in brain, hemopoetic cells and in many types of cancer cells and is invliced in protein trafficking, calcium regulation and apoptosis. The antibody also labels the anterior pharynx defective 1 homolog B in C. elegans.   

The Aph1b-selective antibodies were generated against unique antigenic sequences form near C-terminal end of the Aph1b protein.  The antibodies to Aph1b are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer.  FabGennix Int. Inc., will also provide limited quantities of antigenic blocking peptides for Aph1b antibody and Western blot positive control for Aph1b are also available. FabGennix also provides custom antibody production services for researchers that are looking for high affinity mono and polylconal antibodies in various species. We specialize in making application specific antibodies that are useful in IHC, confocal and other applications where native antigen is detected. For a complete listing of all FabGennix antibodies please visit www.Fabgennix.com.  

Immunogen:            Synthetic peptides taken form with in the amino acids spanning the region of  235-257.  The peptide was post-synthetically modified and coupled to a carrier protein for generating antibodies in rabbit.  The selected peptide was not present in other proteins form the genebank and is 100% conserved in several species including C. elegans.   

Concentration:         Aph1-101AP and Aph1-FITC: IgG concentration 0.6-65 mg/ml in antibody stabilization buffer.

Applications:        Antibody Aph1-101AP and Aph1-FITC are ideal for WB and ELISA applications, other applications have not been tested.  The Aph1-101AP antibody does not cross react to other component of gamma secretase complex nor with other proteins in the PC-Aph1b samples.  The species cross reactivity for these antibodies have not been examined.  The dilutions for this antibody is for reference only, investigators are expected to determine the optimal conditions for specific assay.  WB: > 1:500; IMM & i.p pull-down assays: n.d; IHC n.d.  ELISA <1:10,000.  N.D. does not necessarily means the antibody is not suitable for that application but rather it has not been tested for that particular application.  Investigators using this antibody in applications not listed above can request a complimentary sample of this antibody.

Reactivity:               This antibody detects a single band of approximately 34kDa kDa in PC-Aph1b samples.  The antibody does not cross reacts with other proteins.     

Protocols:                Standard protocol for various applications (WB; IMM and IHC) of this antibody is provided with the product specification sheet, however, FabGennix Inc., strongly recommends investigators to optimize conditions for use of this antibody in their laboratories

For research use only, not for diagnostic or therapeutic use.