Phosphodiesterase Type 1A antibody
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| PD1A-101AP | Pan PDE1A antibody | 0.0 lb |
$245.00
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| P-PD1A | Antigenic petide for PD1A-101AP | Synthetic peptide | Peptide | n/a | 0.0 lb | 100ul |
$125.00
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| PC-PD1A | WB positive control for PDE1A | Protein | 0.0 lb |
$185.00
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| FITC-PD1A | FITC-Conjugated PDE1A antibody | 0.0 lb |
$350.00
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yclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-PDE11) based on their kinetic properties and inhibition to various pharmacological agents. Three different PDE1 genes have been identified (PDE1A, PDE1B, PDE1C). The enzymes of the PDE1A and PDE1B have higher affinity for cGMP than for cAMP, where as, PDE1C has high affinity for both cAMP and cGMP. Calcium-Calmodulin stimulates the activity of each of these enzymes several-fold. The PDE1A gene has been cloned from human, mouse and rat. The PDE1A gene codes a family of proteins (variants) that differ from each other at amino and carboxy termini of the protein. There are three novel PDE1A variants so far identified (PDE1A1; PDE1A3 and PD1A5), PDE1A5 encodes a 519 residue protein , which is different from PDE1A1 by insertion of 14 amino acid residues at the carboxy terminal and is also different from PDE1A3 by a divergent at N-terminus (Miller and Lanfear, 2002). The predicted size of PDE1A gene products ranged between 65-68 kDa. Members of the PDE1A gene have a longer carboxy terminus that accounts for their larger size. The tissue distribution of PDE1A is different from other PDE1 gene family members. In testis PDE1A are highly expressed but indifferent stages of in developing Germ cells. The PDE1A mRNA is also found in round to elongated spermatids, with protein expression in the tails of elongated and maturing spermatids (Yan et. al., 2001).
The PDE1A family-selective antibody was generated against a conserved sequence near the N-terminal that are unique to PDE1A family members. The polyclonal antibody labels a multiple bands in the 64-65 kDa PDE1A1 protein. The antibody also labels various PDE1A variants in rat testis. PDE1A-selective antibody is also available in affinity-purified form for confocal, Western blotting and immunocytochemical analyses. FabGennix Inc. will also conjugate antibodies with fluorescent probes upon request at extra charge. FabGennix Inc. provides PDE family selective, family subtype-selective and family-subtype-variant selective antibodies for detailed analyses of cAMP and cGMP signaling pathways. Some PDE-selective antibodies were generated using cyclic peptide methodology that results in higher titer and specificity (Farooqui et. al., 1991).
FabGennix, Inc., will also provide Western blot positive controls for PDE1A in ready-to-use buffer for easy identification of PDE3 proteins. Limited quantities of antigens are also available. Please enquire for their availability before ordering.
For research use only, not for diagnostic or therapeutic use.
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| FabGennix Product Used | Authors, Year, Journal, Title | Technique used | Comments |
|---|---|---|---|
| PDE1A | Shrivastav A et. al.,
2009
UCSD-Nature Molecule Pages Published online: 9 Sep 2009 | doi:10.1038/mp.a001747.01 Phosphodiesterase 1A, calmodulin dependent |
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