Phosphodiesterase Type 1B antibodies

 

yclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases.  In mammals at least 11 different families of PDEs can be discriminated (PDE1-PDE11) based on their kinetic properties and inhibition to various pharmacological agents.  Three different PDE1 genes have been identified (PDE1A, PDE1B, PDE1C).  The enzymes of the PDE1A and PDE1B have higher affinity for cGMP than for cAMP, where as, PDE1C has high affinity for both cAMP and cGMP.  Calcium-Calmodulin stimulates the activity of each of these enzymes several-fold.  The PDE1B gene has been cloned from human, mouse and rat.  The PDE1B gene codes a family of proteins (variants) that differ from each other at amino and carboxy termini of the protein.  There are at least two novel PDE1B variants so far identified (PDE1B1 and PDE1B2), PDE1B2 encodes a 516 residue protein, which is different from PDE1B1 by replacement of the first 38 residues by an alternative 18 which is predicted to be functionally significant as far its expression in various tissues.  (Miller and Lanfear, 2002).     The predicted size of PDE1B gene products ranged between 65-68 kDa.  Members of the PDE1B gene have a longer carboxy terminus that accounts for their larger size.  The tissue distribution of PDE1B is different from other PDE1 gene family members.  In testis PDE1B is expressed uniformly through out the seminiferous epithelium and in the interstistium in contrast to PDE1A and PDE1C that is expressed in elongated to mature spermatids (Yan et. al., 2001). 

The PDE1B family-selective antibody was generated against a conserved sequence near the N-terminal that are unique to PDE1B family members.  The polyclonal antibody labels a multiple bands in the 64-65 kDa PDE1B1 protein.  The antibody also labels various PDE1B variants in rat testis.  PDE1B-selective antibody is also available in affinity-purified form for confocal, Western blotting and immunocytochemical analyses.  FabGennix Inc. will also conjugate antibodies with fluorescent probes upon request at extra charge.   FabGennix Inc. provides PDE family selective, family subtype-selective and family-subtype-variant selective antibodies for detailed analyses of cAMP and cGMP signaling pathways.  Some PDE-selective antibodies were generated using cyclic peptide methodology that results in higher titer and specificity (Farooqui et. al., 1991). 

FabGennix, Inc., will also provide Western blot positive controls for PDE1A in ready-to-use buffer for easy identification of PDE3 proteins.  Limited quantities of antigens are also available.  Please enquire for their availability before ordering.

For research use only, not for diagnostic or therapeutic use.