Phosphodiesterase Type 4B4

nzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases.  Four genes (PDE4A, B, C and D) make up the PDE4 family.  cAMP-dependent phosphodiesterase type B (PDE4B) family is consists of 4 known splice variants, PDE4B1, B2, B3 and a novel 66 kDa PDE4B4 protein (1, 2).  One or more subtype variants of PDE4B are ubiquitously present in all mammalian cells.  In CNS, all four PDE4B subtype variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation.  Some PDE4B variants are also substrate for PKA-dependent phosphorylation.  Peripheral tissues exhibit differential expression of PDE4B1, B2 and B4 variants; the PDE4B4 (66kDa protein) is the major PDE4B variant expressed in lungs, testis and heart.  In testis PDE4B enzymes are expressed in somatic cells (Sertoli and Leydig cells) while PDE4A is localized in germ cells (2). 

FabGennix Inc. provides PDE family selective, family-subtype-selective and subtype-variant-selective antibodies for detailed analyses of cAMP signaling pathways.  The PDE4B selective antibody was generated using a C-terminal cyclic peptide common to all PDE4B enzymes.  Antibody PD4-200P labels all known PDE4B variants including PDE4B1, B2, B3 and a novel 66kDa PDE4B (PDE4B4) variant ubiquitously present in peripheral tissues.  FabGennix Inc. also supplies ready-to-use Western blot positive controls of all known PDE4B variants for easy identification and quantification purposes.  PDE4B-selective antibody (PD4-200P) is also available in affinity purified (Cat # PD4-201AP) and as an HRP conjugate (PD4-202HRP) for use in confocal, Western blotting and immunocytochemical analyses.  FabGennix Inc. will conjugate antibodies with fluorescent tags (FITC or Rhodamine), make affinity matrices and can provide larger volume aliqouts upon request.

For research use only, not for diagnostic or therapeutic use.