Phosphodiesterase Type 4D

Cyclic AMP-dependent phosphodiesterase type D (PDE4D) family is comprise of 5 variants (PDE4D1, D2, D3, D4 and D5; 1, 2).  One or more PDE4D subtype-variants are ubiquitously present in all mammalian cells.  In CNS all five PDE4D subtype-variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation.  Peripheral tissues also exhibit differential expression of PDE4D variants.  PDE4D1/D2 mRNA levels rise in response to an increase in cAMP (3).  Short-term regulation of PDE4D variants involved PKA, MAP kinases and Erk2 phosphorylation that results in rapid change in their enzymatic activities (2, 4).  Other regulatory mechanism involved protein-protein interactions with cytoskeletal scaffolding proteins (5).  Anti-PDE4D antibodies are ideal tools for studying regulation, expression, phosphorylation, and protein-protein interactions of PDE4D variants employing pull-down immunoprecipitation protocol. 

FabGennix Inc. provides PDE family selective, family subtype-selective and subtype-variant selective antibodies for detailed analyses of cAMP signaling pathways.  The PDE4D selective antibody was generated using unique peptides common to PDE4D members.  The cyclic peptide methodology was used to generate high specificity and high titer antibodies (6).  Antibody PD4-400P/401AP label all known PDE4D variants including PDE4D1, D2, D3, D4 and D5 variants.  FabGennix Inc. also carries Western blot positive controls for all PDE4D variants in ready-to-use buffers for their identification and quantification on Western blots.  PDE4D-selective antibody is also available in affinity-purified form for confocal, Western blotting and immunocytochemical analyses.  FabGennix Inc. will tag these antibodies with fluorescent probes upon request at extra charge. 

For research use only, not for diagnostic or therapeutic use.