PDE4C antibody

Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases.  cAMP-specific phosphodiesterase type-4 (PDE4) family is comprised of 4 genes (PDE4 A, B, C and D) with multiple splice variants generated by RNA splicing and use of alternate initiation sites (1).  The cAMP-dependent protein kinase A modulates the enzyme activity of some PDE4 family members by phosphorylation/dephosphorylation mechanisms (2, 3).   Such short-term regulatory mechanisms are necessary for rapid changes in the cAMP levels in cells.  PDE4-family selective inhibitor, rolipram inhibits all members of the PDE4 family in mM range.  At present PDE4 subtype selective and variant selective inhibitors are not available.  In rodents inhibition of PDE4 enzymes by rolipram attenuated short- and long-term memory impairment produced by the administration of scopolamine and MK801 (3, 4).  Interaction of these enzymes with cellular scaffolding proteins plays important role in PDE4 compartmentalization and cAMP-dependent signaling.  Members of the PDE4A and D subtype variants specifically interact with cytoskeletal proteins with SH3 domains or RACK and AKAP for cellular compartmentalization.  In brain PDE4A, B and D are associated with GPCRs signaling (5, 6).  Little expression of PDE4C was observed in brain, the PDE4C is expressed in other peripheral tissues including testis, white blood cells and heart. 

The PDE4 family-selective antibody was generated against a common sequence near the C-terminal end that is unique to PDE4 family members.  The polyclonal antibody (PD4-300P and PD4-301AP) labels human PDE4C1, C2, and C3 vriants.  Other PDE4 subtypes (A, B and D) had no cross reactivity to this antibody.   FabGennix Inc. provides other PDE family selective, family subtype-selective and family-subtype-variant selective antibodies for detailed analyses of cAMP/cGMP signaling pathways.  The PDE4 selective-antibody (PD4-300P/301AP) was generated using cyclic peptide methodology that results in higher titer and specificity (6).  Antibody PD4-300P and PD4-301AP label all known PDE4C variants including PDE4C1, PDE4C2, PDE4C3.  PDE4-selective antibody is also available in affinity purified form for confocal, Western blotting and immunocytochemical analyses.  FabGennix Inc. will also conjugate antibodies with fluorescent probes upon request at extra charge.  

For research use only, not for diagnostic or therapeutic use.