PDE10A12 antibodies

 

yclic nucleotides are important intracellular second messengers which play important role variety of signal transduction process.  The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases.  One of the many phosphodiesterases that compartmentalized and hydrolyze cAMP and cGMP in to AMP and GMP respectively are phosphodiesterase type 10.  Only one member of the cAMP and cGMP-specific phosphodiesterase type-10 (PDE10) family is cloned and characterized.  The PDE10A gene is expressed in at least two variant forms (PDE10A1, PDE10A2) generated by RNA splicing and or use of alternate initiation sites (1).  The PDE10 gene was first cloned from fetal human lung tissue library using bioinformatics approach (2).  The deduced amino acid sequence contains 779 amino acids (MW 93-95 kDa) including a putative cGMP binding domain near the N-terminal region of the protein.  The catalytic portion is 16-47% identical to other PDEs and PDE10 hydrolyze cAMP and cGMP with Km 0.26 and 7.2 uM with Vmax for cGMP twice as much that for cAMP.  The presence of cAMP inhibits the hydrolysis of cGMP and vise versa.  PDE10A is widely expressed in putamen and caudate nucleus in the brain, thyroid and testis.  Surprisingly the expression of PDE10A was also abundant in Heart (see figure 1).  PDE10 subtype has at least 6 subtype variants (PDE10A2, A3, A11, A12, A13 and A14) produced by either use of alternate initiation codon or by alternate splicing.  PDE10A12 is a 883 amino acid long protein with unique N-terminal sequences and a catalytic domain that is common to all PDE10A variants.  Long term potentiation-associated up-regulation of some PDE10A variants is observed by differential amplification of intron-containing transcripts of the PDE10A variants.   

FabGennix Inc. also provides antibodies to other PDE family members.  The PDE-selective antibodies are also available for family-subtype-selective and family-subtype-variant selective antibodies for detailed analyses of cyclic nucleotide signaling pathways.  The PDE10A12-selective antibodies (PD10A12-121A) are generated against a common sequence near the N-terminal end.  The antignenic peptide is unique to rat PDE10A12 protein and these antibodies label striatum specific PDE10A12 protien.  The polyclonal antibody (PD10A12-121AP) labels a 95 and 98 kDa PDE10A12 variants in PDE10A12 western blot positive control sample.  The PDE10A-specific antiserum has no cross reactivity against any other PDE family members tested so far.  

The PDE10A12-selective antibody (PD10A12-121AP) was generated using proprietary antigenic peptide methodology that results in higher titer and specificity.  PDE10A12-selective antibody is also available in affinity purified form for confocal, Western blotting and immunocytochemical analyses.  FabGennix Inc. will also conjugate antibodies with enzymes, fluorescent probes upon request at extra charge.

For research use only, not for diagnostic or therapeutic use.