PDE11A antibodies

Cyclic nucleotides are important intracellular second messengers which play important role variety of signal transduction process.  The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases.  Recently a new and distinct member (PDE11) of cyclic nucleotide family has been identified and cloned (Fawcett et al., 2000).  The PDE11A exhibit 50% amino acid homology with the catalytic domain of the all other PDEs.   The PDE11A resembles most to the PDE5 family members but has distinct biochemical properties than PDE5.  One of the many phosphodiesterases that compartmentalized and hydrolyze cAMP and cGMP in to AMP and GMP respectively are phosphodiesterase type 11. The human PDE11A contains an open reading frame encoding a 490 amino acid protein (55-56 kDa).  The PDE11A has an N-terminus GAF domain homologous to other signaling molecule as found in PDE2, PDE5, PDE6, PDE10 this potentially represent an allosteric cGMP and other small signaling molecules.  The PDE11A hydrolyzes both cGMP and CAMP with Km values of 0.52 uM and 1.04 uM range.  The PDE11A is sensitive to non-selective PDE inhibitor IBMX as well as zaprinast and dipyrimadole, inhibitors that are generally selective for cGMP-specific enzymes.  PDE11A expression is diverse and is found in high levels in skeletal muscle, prostate, kidney, liver, pituitary and testis.  At least three variants that may occur in vivo based on the three transcripts identified in various tissues ranging in size from 10.5 kb, 8.5 and 6.0 kb.  Western blotting with PDE11A antibody labels at least three PDE11A variants 79 kDa, 65 kDa and a 57 kDa. 

FabGennix Inc. has made two antibodies to the PDE11A protein, PD11A-100P/PD11A-101AP epitope is close to the N-terminus of the proteins; while (PD11A-110P/PD11A-112AP) epitopes lie close to the C-terminal end of the protein.  FabGennix International Inc., also provides antibodies to other PDE family members.  The PDE-selective antibodies are PDE-selective, family subtype-selective and family-subtype-variant selective antibodies for detailed analyses of cyclic nucleotide signaling pathways.  The PDE11A-selective antibodies (PD11A-100P and PD11A-101AP) are generated against a common sequence near the 115-129 amino acids down stream from N-terminus and PD11A-110P and PD11A-112AP is against 454-468 position.  The antigenic peptides were used for conjugation to KLH for generation of PDE11A antibodies.   The polyclonal antibody (PD11A-100P/101AP) labels an 80kDa, 65 kDa and a 57 kDa proteins in various tissues.  PDE11A-specific antiserum has no cross reactivity against any other PDE family members tested so far.   PDE11A-selective antibody is also available in affinity purified form for confocal, Western blotting and immunocytochemical analyses.  FabGennix Inc. will also conjugate antibodies with enzymes, fluorescent probes upon request at extra charge.

For research use only, not for diagnostic or therapeutic use.