Receptor Interacting Protein
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| RIP-101AP | RIP antibodies | Rabbit | AP | r, m, h | 100.0 ug | 150-500 ul |
$245.00
|
| RIP-112AP | RIP antibodies | Rabbit | AP | r, m, h | 100.0 ug | 150-500 ul |
$245.00
|
| RIP-121AP | RIP antibodies | Rabbit | AP | r, m, h | 100.0 ug | 150-500 ul |
$245.00
|
| FITC-RIP100 | FITC-conjugated RIP-101AP | Rabbit | FITC-AP | r, m, h | 100.0 ug | 150-500 ul |
$350.00
|
| FITC-RIP110 | FITC-conjugated RIP-112AP | Rabbit | FITC-AP | r, m, h | 100.0 ug | 150-500 ul |
$350.00
|
| FITC-RIP120 | FITC-conjugated RIP-121AP | Rabbit | FITC-AP | r, m, h | 100.0 ug | 150-500 ul |
$350.00
|
| P-RIP140 | Antigenic blocking RIP-1, 2 and 3 peptides | n/a | Synthetic peptide | n/a | 250.0 ug | 100 ul |
$125.00
|
| PC-RIP140 | WB positive control for RIP140 | n/a | Protein in ready-to-use buffer | n/a | 0.0 ug | 150 ul |
$185.00
|
The nuclear receptor transcriptional activity is regulated by coregulators such as receptor interacting protein 140 (RIP140) by providing an bridging apparatus between nuclear receptor and the transcription machinery. It has recently been shown that Orphan receptor SF-1, which regulates the cAMP stimulated expression of human steroidenic acute regulatory protein gene DAX-1, interacts with RIP140 (1). RIP140 also regulated the target gene expression in response to corticosteroid hormone receptor (glucocorticoid receptor, GR) stimulation. The ligand binding domain of the GR receptor is sufficient for the RIP140 interaction. It has been suggested that both N and C terminal regions of the RIP140 interacts and binds to the GR receptor, thereby modulate the transactivation transcriptional activity of the receptor (2). RIP140 encodes a histone deactylase (HDAC) inhibitor–sensitive responsive activity (3). These interactions are the result of direct contact of RIP140 and HDAC1 and HDAC-2. The HDAC interaction domain is mapped to its N-terminal domain between amino acid 78 and 303 (3). Cloned RIP140 is a 4307 bp long and the deduced amino acid sequence contain a 1161 amino acid nuclear protein (MW 140 kDa) including a putative HDAC domain near the N-terminal region of the protein. RIP 140 is widely expressed in nuclear fractions of most of the glucocorticoid responsive cells including CNS, adrenals, kidney etc.
FabGennix International Inc. has produced three epitope-specific antibodies for RIP140. The N-terminal epitope (Cat # RIP140-101AP) covers the HDAC binding domain, the mid-region epitope (Cat # RIP140-102AP; around aa 750-770) and a C-terminal epitope (Cat # RIP140-103AP). The polyclonal antibodies to RIP140 label a 140 kDa nuclear protein from nuclear extracts of COS1 cells transfected with human RIP140 cDNA. The RIP140-specific antiserum has no cross reactivity against any other protein tested so far. FabGennix Inc. also provides Western blot positive controls for nuclea RIP140 protein in ready-to-use buffer. FabGennix Int. Inc., has made antibodies to other signal transduction targets such as PDE family members, mer tyrosine kinase (MERTK), calmodulin kinase g, various G-protein coupled receptors (GPCRs), chemokine receptors (CCRX1-CCRX4), Sec23 protein, several retinal proteins and antibodies to various Drosophila proteins.
The RIP140-selective antibodies were generated using circular peptide methodology that results in higher titer and specificity. RIP140-selective antibody is also available in affinity purified form for confocal, Western blotting and immunocytochemical analyses. FabGennix Inc. will also conjugate antibodies with enzymes, fluorescent probes upon request at extra charge.
For research use only, not for diagnostic or therapeutic use.
Data Sheets
MSDS
General Information
Citations
Search FabGennix Product Citation Data Base
Search