Ras homolog enriched in striatum

Rhes, Ras homolog enriched in striatum, belongs to the RASD subfamily of the Ras-related GTP-binding protein superfamily. By responding to extracellular signals, these proteins regulate intracellular signaling pathways, thereby controlling essential cellular functions such as cell growth, gene transcription, and cytoskeleton organization and also pathways involved in synaptic plasticity, learning and memory (1). Rhes, located on mouse chromosome 8, is a product of thyroid hormone-regulated gene during brain development and it regulates signal transduction from G protein-coupled receptors (2). It is implicated in cAMP/PKA signaling pathway- Rhes has been shown to inhibit the cAMP/PKA pathway by thyroid-stimulating hormone (3). Furthermore, Rhes modulates dopamine signaling in the striatum- It is essential for correct dopamine-mediated GTP binding (4).

Studies investigating the role of Rhes have shown that mice that are deficient in this protein were smaller than the wild type mice (1). These mice also showed behavioral abnormalities such as gender-dependent increase in anxiety levels and lack of clear motor coordination. Such studies also showed that absence of Rhes modulated cAMP/PKA signaling in projection neurons by increasing golf protein levels (2). Rhes is expressed in brain areas that receive dopaminergic input, such as striatum. Therefore, its mRNA expression is controlled by dopamine and its expression is reduced under conditions of dopamine supersensitivity (5). Rhes has also been shown to bind huntingtin and mutant huntingtin protein (6). Expansion of huntingtin is usually seen in patients with Huntington’s disease. Rhes is approximately a 70kDa protein (6), higher than the predicted MW of 38kDa (317 amino acids).

The Rhes antibodies were generated using peptide corresponding to mouse Rhes protein. Rhes antibodies are affinity purified over immobilized antigen based affinity chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. FabGennix Int. Inc. will provide limited quantities of antigenic blocking protein for competition assays involving Rhes antibody. Antibodies to Rhes (Rhes-101AP) will label ~70kDa protein in Western blot positive control for Rhes and several other tissues. FabGennix Inc. will conjugate this and other antibodies from its catalog to either secondary enzymes (alk-Pase or HRP) or fluorescent probes at a nominal cost upon request. FabGennix also provides custom antibody production services for researchers that are looking for high affinity mono and polylconal antibodies in various species. We specialize in making application specific antibodies that are useful in IHC, confocal and other applications where native antigen is detected. For a complete listing of all FabGennix antibodies please visit www.Fabgennix.com.  

For research use only, not for diagnostic or therapeutic use.