Dopamine D1 Receptor

ive types of dopamine receptors designated as (dopamine D1-dopamine D5) have been characterized based on cDNA cloning, ligand binding and behavioral responses to antagonist/agonists.  Molecular cloning of dopamine receptors have identified a family of closely related genes that encodes 5 proteins with some splice variants exhibiting high degree of amino acid homology (45%-65%) all in the molecular weight range of 40-60 kDa.  Individual members of the dopamine receptor family have predicted secondary structure having 7 transmembrane spanning helical domains characteristic feature of G-protein coupled receoptors.  Stimulation of dopamine D1 receptor led to Gs coupled adenylate cyclase activation resulting in significant increase in camp levels.  All dopamine receptors proteins are glycosylated at or near the C-terminus and are present on either cell surface or in intracellular sites.  Some transporters exhibit dynamic trafficking between intracellular storage sites and plasma membranes in response to various stimuli.  Dopamine receptors are believed to be associated with other proteins including members of the dopamine receptors and opiod receptors resulting in a significant increase in their apparent molecular weight.   

The dopamine receptor family-selective antibodies were generated against unique C-terminal peptides characteristics of a particular dopamine receptor.  FabGennix Inc. has   generated highly specific rabbit anti-dopamine D1 receptor polyclonal antibodies utilizing unique N-terminal sequences.  These antibodies have been fully characterized for cross reactivity with in the dopamine receptor family and with other cellular proteins.  FabGennix Inc. has produced antibodies to multiple epitopes on the same protein that will facilitate studies utilizing interspecies cross reactivity.  

FabGennix Inc. provides dopamine D1 Western blot positive control in “ready-to-use” SDS-PAGE sample buffer.  The dopamine D1 positive control appears as a diffuse band of 49-55 kDa and co-migrates with rat brain dopamine D1 receptor protein on a 10% SDS-PAGE.

For research use only, not for diagnostic or therapeutic use.