Terminal Deoxynucleotidyl Transferase

Catalog#	Product Description	Host Species	Nature	Cross reactivity	Quantity	Volume	Price
TdT-101AP	TdT antibodies	Rabbit	AP	r, m, h, c, monk	100.0 ug	150-500 ul	$245.00
FITC-TdT	FITC-conjugated TdT antibodies	Rabbit	FITC-AP	r, m, h, c, monk	100.0 ug	150-500 ul	$350.00
P-TdT	Antigenic blocking peptide	n/a	Synthetic peptide	n/a	250.0 ug	100 ul	$125.00
PC-TdT	WB positive control for TdT	n/a	Protein in ready-to-use buffer	n/a	0.0 ug	150 ul	$185.00

Terminal Deoxynucleotidyl Transferase (TdT). TdT is a DNA polymerase located in the cell nucleus which catalyses the polymerization of deoxynucleotides at the 3’ hydroxyl ends of oligo or polydeoxynucleotide initiators and functions without a template. The Terminal Deoxynucleotidyl Transferase (TdT) enzyme involved in DNA polymerization and is localized in the nucleus of several hematopoeitic cells such as prothymocytes, precursor T and a subset of B cells. The TdT protein is a 58 kDa nuclear protein. The expression of TdT has been known as a nuclear maker for the hemopeotic cells for many years (1). The conspicuous absence of expression and activity of TdT clearly distinguishes B cell development in the fetus from that in adult bone marrow. The TdT is expressed in most of cases of acute lymphatic leukemia (ALL), and non Hodgkinson lymphoma (NHL), in a subset of chronic and acute myeloid leukemia (CML and AML), acute non-lymphocytic leukemia (ANLL; 1, 2). Detection of nuclear expression of TdT is a valuable method for detection of various type of leukemia and monitoring minimal residual leukemic cells. The DNA polymerase, TdT, is involved in the rearrangement of the T-cell receptor genes in early thymocytes and in rearrangement of of the immunoglobulin genes during B-cell development. These rearrangements of the T-cell receptor genes and the immunoglobulin genes are performed in the nucleus and are lost upon maturation of the immune cells. Detection of TdT by flow cytometry and by IHC can be useful in detecting a small percentage of leukemic cells, in cases such as minimal residual T-cell acute lymphoblastic leukemia (3). Other antibodies that are used to detect residual T-cell ALL are combination of Anti-TdT with CD1a, CD2, CD5 and CD7 antibodies. The sensitivity of detecting smaller percentage of cell expressing TdT can be enhanced by double or triple staining with Anti-TdT and a combination of anti-CD antibodies. Antibodies used for double staining of AML cells are combination of Anti-TdT and Anti-HLA-DR, CD13, CD33, CD34 and CD45 antibodies. The anti-TdT antibodies label a 58 kDa nuclear protein in Western blot positive control and in bone marrow cells.

FabGennix Inc. provides Western blot positive controls for the TdT in ready-to-use buffer for applications in Western blot analyses. The TdT antibodies were (TdT-100P/101AP) were generated using cyclic peptide methodology that results in higher titer and specificity (6). Antibody TdT-100P and TdT-101AP also label nuclear TdT protein in CML, AML and ANLL samples in immuno-histochemistry applications. TdT-selective antibody is also available in affinity purified form for confocal, Western blotting and immunocytochemical analyses. FabGennix Inc. will also conjugate antibodies with fluorescent probes upon request at extra charge.

For research use only, not for diagnostic or therapeutic use.

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Terminal Deoxynucleotidyl Transferase

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