CD3 Epsilon

Catalog#	Product Description	Host Species	Nature	Cross reactivity	Quantity	Volume	Price
CD3-101AP	CDEpsilon antibodies N-epitope	Rabbit	AP	r, m, h, others	100.0 ug	200ul	$245.00
CD3-112AP	CD3 mid-region epitope abs	Rabbit	AP	r, m, h, others	100.0 ug	200ul	$245.00
CD3-121AP	CD3 C-epitope antibody	n/a	AP	n/a	100.0 ug	200 ul	$245.00
CD3-131AP	CD3 extreme C terminal Antibody	Rabbit	AP	r,m,h	100.0 ug	200ul	$245.00
P-CD3	Blocking peptides (inquire)	Rabbit	peptide	r, m, h, others	250.0 ug	100ul	$145.00
CD3-FITC	FITC-conjugated antibodies	Rabbit	AP	r, m, h, others	100.0 ug	150-500 ul	$350.00

The antigen receptor on human T lymphocytes consists of two variable immunoglobulin-like glycoproteins, alpha and beta or TCR-gama and delta, which occur in association (noncovalently) with three invariable T3 membrane proteins (CD3-gamma, CD3-delta and CD3 epsilon). In humans two of these proteins, T3-gamma and T3-delta, are glycoproteins of relative molecular mass (Mr) 25,000 (25K) and 20,000 (20K), respectively, while the third, T3-epsilon, is a 20K non-glycosylated protein. A keystone of the antigen-specific immune response is the T-cell receptor (TCR)/CD3 complex. CD3 antigen has gamma, delta, epsilon, and theta chains. The CD3 complex consist of various chains (alfa, beta, gamma, theta, epsilon and delta) mediates signal transduction in T cell receptor cells. The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer co-expressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The infection of CD3 positive cells with HTLV led to a decrease in CD3 antigen expression. The onset of this phenomenon coincided with a decrease of CD3γ followed by the subsequent progressive reduction in CD3δ, then CD3ε and CD3ζ mRNA. This decrease in CD3 expression is a direct result of epigenetic control regulation of CD3 genes as determined by DNAse I hyper sensitivity assay (DHS) (1).

Antibodies to CD3 epsilon chain is a glycosylated protein of 290 amino acids with a single trans-membrane spanning domain residing between 127-149 with an apparent MW of 20 kDa. The epsilon chain of CD3 complex is non-glycosylated and is involved in rapid turnover of phosphotidylserine and accumulation of intracellular Ca after antigen receptor activation on T cell. The CD3 epsilon gene consist of nine exons and three mini exons of 21, 15 and 18 bases, the epsilon gene is transcribed form weak, tissue non-specific TATA less promoter (2).

The CD3E-selective (CD3 epsilon chain) antibodies were generated against peptide form unique region of the CD3 gene that is not present in other CD3 antigen chains or other cellular proteins. The antigenic sequence was common in several species but distinct from other CD antigens. FabGennix Inc. has generated epitope specific anti-CD3E mono-epitope-specific antibodies utilizing cyclic peptide methodology. The Anti-CD3 antibodies have been fully characterized for cross reactivity with other cellular proteins using Western blot analyses and IHC applications. CD3 antibodies are also available in FITC-conjugated form. Limited quantities of antigenic blocking peptide and Western blot positive controls in ready-to-use buffer for CD3 are also available. FabGennix International Inc., will conjugate this antibody to fluorophores and to secondary enzymes at a nominal charge. FabGennix also carry a wide selection of antibodies against CD antigens for a complete listing please visit www.FabGennix.com.

Immunogen: Synthetic peptides taken form CD3 epsilon chain corresponding to aa 159-180; from amino acids 186-206 corresponding to the following sequence (DYEPIRKGQRDLYSGLNQRRI),was selected from the unique region of the CD3 protein, peptide was post-synthetically modified to achieve highest antigenicty before used for coupling to KLH using heterobifunctional cross linker for immunogen preparation.

Concentration: CD3-101AP, 112AP, 121AP and 131AP antibodies have a concentration range of 0.6-0.68mg/ml in antibody stabilization buffer.

Applications: Antibody dilution 1:2,000 for ELISA or DOT blot assy. W.B: Antibody dilution 1:500-1,000 for WB using PC-CD3. IMM: n.d; IHC dilutions in DiluObuffer at 1:200-250

Reactivity The antibodies CD3-101AP antibody labels a 20kDa band of CD3 in PC-CD4 samples using antibody dilution at 750 in diluObuffer.

Protocols: Standard protocol for various applications (Western blot; immunoprecipitation and immunohistochemistry) of this antibody is provided with the product specification sheet, however, FabGennix Inc. recommends investigators to optimize conditions.

For research use only, not for diagnostic or therapeutic use.

Data Sheets
CD3 Epsilon

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