Runt-related Transcription faactor3

Accession #/Synonyms: NP_001026850; RUNX3 protein, Runt-related transcription factor 3 isoform 1 .PEBP2alfaC.  

Runt-related Transcription factor1 (Runx1) belongs to the family of Runt-related to a transcription factors comprising of 3 subtypes (Runx1-3).  Regulation of cell proliferation by RUNX proteins represents an evolutionarily conserved activity.  All vertebrates have three Runx genes and all three genes have P1 and P2 promotors. The Runx3 P1 and P2 promoter regions showed that a small (around 600 bp) P1 element could direct cell-type-specific expression with fidelity (1).   Runt-related transcription factor 3 (RUNX3) is a candidate tumor suppressor gene that is downregulated in various cancers that paly important rol es in cell proliferation and apoptosis. The mammalian AML/CBFalpha runt domain (RD) transcription factors regulate hematopoiesis and osteoblast differentiation. Like their Drosophila counterparts, most mammalian RD proteins terminate in a common pentapeptide, VWRPY, which serves to recruit the corepressor Groucho (Gro). The evolutionary conserved Runt domain harbors DNA binding and heterodimirizing activities.   The PEBP2/CBF is a heterodimeric transcription factor composed of alpha and beta subunits. The alpha subunits have PEBP2alfaA and PEBP2 alfaB and PEBP2 alfa C.  
the Runx3 gene codes a 51kDa protein, a heterodimier for Runx3 and a beta subunit forms a complex that binds to the core DNA sequence 5’-PYGPYGGT-3’ found in a number of enhancer and promotors.  The complex of Runx3:beta subunit can activate or suppress the transcription and also can inter actis with other transcription factors.  Runx3 is a tumor suppressor protein and  its gene is frequently transcriptionally silenced in different kind of cancer.  .  

Runx3- selective antibodies were generated against a peptide taken form N-terminal region spanning 200-250 amino acids. The 20mer peptide was conserved in Runx3 isoform 1 and isoform 2 of Runx3.  The antibodies do not cross react to other Runx subtypes. The anti-Runx1-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions.  The Runx1antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage.  FabGennix Int. Inc. provides Runx1Western blot positive control samples in “ready-to-use” SDS-PAGE sample buffer.  Limited quantities of antigenic blocking peptide for Runx1antibody is also available, please inquire before ordering.  FabGennix Int. Inc., will also conjugate this antibody to fluorophores and other secondary enzymes at a nominal price. FabGennix Int. Inc., provides custom antibody production services for researchers that are looking for high affinity mono and polyclonal antibodies in various species. We specialize in making application specific antibodies that are useful in IHC, confocal and other applications where native antigen is detected. For a complete listing of all FabGennix antibodies please visit www.Fabgennix.com. 

For research use only, not for diagnostic or therapeutic use.