Lactoferrin human

 

actoferrin, an iron-binding glycoprotein with an approximate molecular weight of 80 kDa.  The protein has two iron binding domains each housing one Fe(3+) and the synergistic CO(3)(2-) ion (1).  The crystal structure of differic form of human lactoferrin at 2.2A resolution exhibit 5330 protein atoms, 2Fe+2 , 2CO3-2 and 98 carbohydrate atoms (1).

Lactoferrin is absorbed from intestine by apical; side of the membrane and localized to the nuclei (2).  Anti-lactoferrin antibodies were detected in some patients with rheumatoid arthritis and systemic lupus erythematousus and other muscle diseases (3).  Intravenous infusion of lactoferrin is also protective against lethal dose of E coli induce bacterimia by a mechanism that down regulate neutrophil TNF alfa secretion (4).  Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency-induced anemia. Lactoferrin has been shown to have a role in immune system and in early development of embryo. A specific receptor for lactoferrin binding has been implicated in human fetal intestine (5).  Early embryonic localization of lactiferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain. 
FabGennix International Inc. has produced affinity purified antibdies to human and bovine lactoferrin proteins.  These antibodies are generated in rabbit using purified human or bovine lactoferrin proteins.  The lactoferrin antibodies were then affinity purufied using immobilized antigenic proteins and pre-adsorbed on an immobilized heterologus lactoferin matrix to remove the cross-reacting antibodies.  Limited quantities of blocking protein is also available (please inquire).   FabGennix, Inc., will also provide Western blot positive controls for most of these antibodies in ready-to-use buffer for easy identification of respective proteins.  FabGennix International Inc. will also label lactoferrin antibdoies to fluorophores or to alkaline-phoshpatase or horseraddish perxidase for a nominal price. 

For research use only, not for diagnostic or therapeutic use.