Cytokeratin-7 (Cytk7) is a member of fibrous structural proteins known as cytokeratins or keratins. Proteins in this group are a key component in the outer layer of human skin, hair and nails (1). They are heteroteramers composed to two type I and type II subunits. Type I cytokeratins are represented by the type I intermediate filaments of the intracytoplasmatic cytoskeleton, whereas type II cytokeratins comprise the type II intermediate filaments of the intracytoplasmatic cytoskeleton (2). Type II cytokeratins consists of basic or neutral proteins. Cytk7 is a basic cytokeratins, located at human chromosome 12q12-q13. It is known to stimulate DNA synthesis in cells; it is also known to block interferon-dependent interphase (3). Studies have reported that Cytk7 can be used as a marker to distinguish a normal tissue from a lesional tissue. In one study, it was used to determine the primary site of metastatic lesion (4). Ctyk7 may also be helpful in distinguishing a large cell neuroendocrine carcinoma from a small cell lung carcinoma by analyzing and comparing the expression of this protein in both carcinomas (5).
Cytk7 is found on many glandular and transitional epithelia but not on stratified squamous epithelia. More specifically, it is expressed in the epithelial cells of ovary, lungs and breast but it is absent in the epithelial cells of colon, prostrate or GI tract (6). Therefore, expression of Cytk7 in colon could be used as a positive marker for colorectal carcinogenesis (7). Cytk7 was also implicated in regulating the translation of human papillomavirus type 16 E7 mRNA (3). Cytk7 is approximately a 59kDa protein (469 amino acids).
The Cytk7 antibodies were generated using peptide corresponding to human Cytk7 protein. Cytk7 antibodies are affinity purified over immobilized antigen based affinity chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. FabGennix Int. Inc. will provide limited quantities of antigenic blocking protein for competition assays involving Cytk7 antibodies. Antibodies to Cytk7 (Cytk7-701AP) will label ~59kDa protein in Western blot positive control for Cytk7 (PC-Cytk7) and several other tissues. FabGennix Inc. will conjugate this and other antibodies from its catalog to either secondary enzymes (alk-Pase or HRP) or fluorescent probes at a nominal cost upon request. FabGennix also provides custom antibody production services for researchers that are looking for high affinity mono and polyclonal antibodies in various species. We specialize in making application specific antibodies that are useful in IHC, confocal and other applications where native antigen is detected.
For research use only, not for diagnostic or therapeutic use.
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Cytokeratin 7
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