The Proopiomelanocortin (POMC) is expressed in the arcuate nucleus of the hypothalamus (ARC) and the commissural nucleus of the solitary tract (cNTS) is post-translational processed to produce two melanocortin receptor ligands, alpha- and gamma-melanocyte-stimulating hormone (MSH). These endogenous peptides have long been reported topossess anti-inflammatory effects in many experimental modelsof acute and chronic inflammation, including experimental boweldisease, allergy, and chronic (mycobacterium-induced arthritis)and systemic inflammation (endotoxemia) (1). Melanocortins are ligands for a subgroup of GPCRs positively coupled to Adenylate cylcases, termed as melanocortin receptor (MC1-R, MC2R, MC3R, MC4R amd MC5R). These receptors are widely expressed at varied density throughoutthe body. Specific physioological actions have been implecated to specific MC recpetors such as skin pigmentation to MC1C, stimulation of adrenal steriodogenesis to MC2R, obesity ralted effects to MC4R and anti-inflammatory effects to MC3R (2)
Two melanocortin receptors (MC3R, MC4R) are expressed in brain regions receiving projections of POMC fibers, most of which also receive projections from a population of arcuate neurons that co-express neuropeptide Y (NPY) and the MC3R/MC4R antagonist agouti-related peptide (AgRP). Recent dta suggest that MC3R knock out mice exhibit reduced linear growth and lean mass and are hyperleptinemic and hyperinsulinemic. The MC3R knock out mice did not show type 2 diabetes contract to a common finding in MC4R knock out mice (3).
The Anti-MC3R-selective antibodies were generated against 3 unique epitopes from the MC3R receptor sequence that are conserved in several species. The peptides were post-synthetically modified to enhance antigenicty and conjugation to a carrier protien. The N-terminal, mid-refion and C-terminal epitopes are unique to MC3R receptor only. These antibodies do not cross react with other MC receptors. The polyclonal antibodies strongly labels a 55-60 kDa protein in PC-MC3R samples. Anti-MC3R-selective antibodies are also available in affinity-purified form for Immunoprecipitation, immunohistochemistry and Western blotting analyses. FabGennix Inc. will also conjugate antibodies with fluorescent probes upon request at a nominal extra charge. FabGennix, Inc., will also provide Western blot positive controls for most of these antibodies in ready-to-use buffer for easy identification of respective proteins. Limited quantities of antigenic peptides are also available. Please enquire for their availability before ordering. FabGennix Inc. also provides antibodies against several other GPCRs, for a complete listing please visit GPCR listing under products at www.fabgennix.com . FabGennix Inc., employs cyclic peptide methodology for generating antibodies, which results in higher titer and specificity (4).
For research use only, not for diagnostic or therapeutic use.
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