Phospho Transient Receptor Potential
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| PTRP-140AP | Phospho-TRP antibodies | Rabbit | AP | D. Melanogaster | 100.0 ug | 150-500 ul |
$285.00
|
| FITC-PTRP | FITC-conjugated antibodies | Rabbit | FITC-AP | D. Melanogaster | 100.0 ug | 150-500 ul |
$350.00
|
| P-PTRP | Antigenic blocking peptide | n/a | Synthetic peptide | n/a | 250.0 ug | 100 ul |
$145.00
|
| PC-PTRP | WB positive control for PTRP | n/a | Protein in ready-to-use buffer | n/a | 0.0 ug | 150 ul |
$195.00
|
The ion channels carrying the stimulus-activated receptor current in sensory neurons have been identified and the mechanism of their activation elucidated, for a variety of sensory systems and for species ranging from worms and flies to humans. In physiological terms the Transient Receptor channels should be a non-selective cation channel that is permeable to all the alkali cations and to many divalent cations. The channel has a particularly high calcium permeability, passing Ca2+ at least 10 times better than Na+ at the same time, Ca2+ in millimolar concentrations acts as a partial blocker, inhibiting current by monovalent ions. Most eukaryotic genomes include a substantial repeat-rich fraction termed heterochromatin, which is concentrated in centric and telomeric regions. The repetitive nature of heterochromatic sequence makes it difficult to assemble and analyze. To better understand the heterochromatic component of the Drosophila melanogaster genome, the entire transposable region was annotated for a whole-genome shotgun sequence assembly. The shot gun sequence assembly analyses predicted 297 protein-coding genes and six non-protein-coding genes, including known heterochromatic genes, and regions of similarity to known transposable elements (1). One of these gene code for a protein transient receptor potential (Trp). The transient receptor potential gene is referred to in FlyBase by the symbol trp (CG7875, FBgn0003861). It has the cytological map location 99C6-99C7. Its sequence location is 3R:25740242: 25746011. There are at least one more subtype is known to exist as TrpA1.
Trp is a MW amino acid proteinTrp is a calcium ion channel component and its function is described in modulation of calcium channel activity. It also regulates calcium calmodulin and intracellular ligand-gated calcium channel and store-operated calcium channels. The physiological functions associated with Trp include modulation in the light activated voltage-gated channel activity, calcium ion transport in response to light stimulus and in light induced release of cytosolic sequestered calcium ion. Physiologically, trp involvement is also studied in calcium-mediated signaling; phototransduction; phospholipase C inhibition. There are 29 alleles are reported in literature, the phenotypes of these alleles are annotated with: photoreceptor; photoreceptor cell; antenna; rhabdomere; ommatidium; retina. It has one annotated transcript and one annotated polypeptide. Trp undergoes phosphorylation for activation at position. There are number of human homolog for the fly Trp channels including TRPN1, TRPV4, TRPML3 and TRPA1 (2).
FabGennix Int. Inc., has produced anti-TRP antibodies against unique peptide fro Trp sequence. FabGennix Inc employs cyclic peptide methodology for generating antibodies, which results in higher titer and specificity. The anti-trp antibodies are affinity purified using immobilized antigen affinity chromatography and are characterized for Western and immunohistochemical applications. Anti-trp antibodies do not cross react to other drosophila proteins. Phosphospecific antibodies are produced by using peptides containing phosphorylated. The phosphospecific antibodies are isolated using differential affinity chromatography using phosphorylated and non-phosphorylated antigenic peptides. The non-phopsho-specific antibodies to Trp are adsorbed and removed form the final preparation using non-phosphorylated peptide affinity matrix. Th phosphor and non-phospho-sepcific Trp antibodies were for Western and IHC applications. FabGennix Inc. will conjugate both pan and phosphor-specific Trp antibodies to fluorescent probes or secondary enzymes at a nominal charge upon request. FabGennix Inc. also a wide range of fruit fly antibodies in stock, for a complete listing of these antibodies, please visit www. Fabgennix.com. FabGennix, Inc., will provide Western blot positive controls for most of these antibodies in ready-to-use buffer for easy identification of respective proteins. Limited quantities of antigenic blocking peptides are also available. Please inquire for their availability before ordering.
For research use only, not for diagnostic or therapeutic use.
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