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Punt

Catalog# Product Description Host Species Nature Cross reactivity Quantity Volume Price
Punt-101AP Punt antibodies Rabbit AP D. Melanogaster 100.0 ug 150-500 ul $245.00
Punt-112AP Punt antibodies Rabbit AP D. Melanogaster 100.0 ug 150-500 ul $245.00
FITC-Punt100 FITC-conjugated Punt-101AP Rabbit FITC-AP D. Melanogaster 100.0 ug 150-500 ul $350.00
FITC-Punt110 FITC-conjugated Punt-112AP Rabbit FITC-AP D. Melanogaster 100.0 ug 150-500 ul $350.00
P-Punt100 Antigenic blocking peptide for Punt-101AP n/a Synthetic peptide n/a 250.0 ug 100 ul $125.00
P-Punt110 Antigenic blocking peptide for Punt-112AP n/a Synthetic peptide n/a 250.0 ug 100 ul $125.00
PC-Punt WB positive control for Punt n/a Protein in ready-to-use buffer n/a 0.0 ug 150 ul $185.00
F
ruit fly (Drosophila melanogaster) ovaries contains two set of germline stem cells surrounded by a group of highly differentiated somatic cells that express genes for two phenotypes (hedgehog & wingless).   The TGF beta super family member, decpentaplegic (dpp) or its homologue BMP2/4 is specifically required for maintenance and promote its cell division in the female germline (1, 2). The Signaling by TGF beta-related factors requires ligand-induced association between type I and type II transmembrane receptors that have endogenous serine/threonine kinases activity. In Drosophila, the type I receptor encoded by the thick veins (tkv) gene and the type II receptor encoded by the punt (put) gene. These receptors mediate signaling by decapentaplegic (dpp), a member of the bone morphogenetic protein (BMP) subgroup of TGF beta-type factors.  Over expression or mutation in dpp suppress germline stem cell differentiation. The Drosophila punt gene encodes a type II serine/threonine kinase TGF-beta/Dpp (Decapentaplegic) receptor.  Dpp actions are mediated by its receptor Punt and Saxophone. There are 5 down stream component in the dpp signaling cascade required ot block the development of various organelles including salivary glands, these are Mothers against dpp (Mad), Medea (Med) Schnurri (Shn). Punt signaling is also responsible for calcium gradient formation during D. melanogaster development (3). Punt gene encodes for a homolog of vertebrate type II receptor and Punt, like thick veins (Tkv) is essential for in vivo dpp-dependent patterning process. No Dpp-dependent signal processing is apparent in the absence of Punt ot Tkv suggesting that both receptors acts in concert to transduce Dpp signaling.     
The Anti-Punt-selective antibodies were generated against conserved sequences near the N-terminus and the C-terminal end of the protein that are unique to Punt protein.  The Punt-selective antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded epitope-specific antibodies. The Punt antibodies label a 63 kDa protein in D. melanogaster extracts. Anti-Punt-selective antibodies are also available in affinity-purified form for confocal, Western blotting and immunocytochemical analyses. FabGennix Inc. will also conjugate antibodies with fluorescent probes upon request at extra charge.  
FabGennix Inc. also provides several antibodies against proteins that are involved Drosophila melanogaster research such as several Anti-PDE antibodies, Anti-Wishfulthinking, Anti-Baboon, Anti-Shadow, Anti-Shade, Anti-SARA anchor protein, Anti-Phantom and Anti-Saxophone protein. FabGennix Inc employs cyclic peptide methodology for generating antibodies, which results in higher titer and specificity (6). FabGennix, Inc., will also provide Western blot positive controls for most of these antibodies in ready-to-use buffer for easy identification of respective proteins. Limited quantities of antigens are also available. Please enquire for their availability before ordering.

For research use only, not for diagnostic or therapeutic use.

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Punt

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